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Literature summary for 3.2.1.4 extracted from
- Characterization and substrate specificity of an endo-beta-1,4-D-glucanase I (avicelase I) from an extracellular multienzyme complex of Bacillus circulans (1995), Appl. Environ. Microbiol., 61, 959-965.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cellobiose | inhibits hydrolysis of carboxymethylcellulose and avicel | Niallia circulans |  |
| Cu2+ | - |
Niallia circulans | |
| Hg2+ | - |
Niallia circulans | |
| PCMB | 0.4 M, 60% inhibition | Niallia circulans | |
| SDS | - |
Niallia circulans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Niallia circulans |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 2 mM, 40% stimulation | Niallia circulans | |
| Mg2+ | stimulates | Niallia circulans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
gel filtration | Niallia circulans |
| 82000 | - |
1 * 82000, SDS-PAGE | Niallia circulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Niallia circulans | - |
F-2 | - |
| Niallia circulans F-2 | - |
F-2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Niallia circulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl cellobioside + H2O | - |
Niallia circulans | ? | - |
? | |
| avicel + H2O | - |
Niallia circulans | ? | main product is cellobiose | ? | |
| carboxymethylcellulose + H2O | - |
Niallia circulans | additional information | - |
? | |
| carboxymethylcellulose + H2O | - |
Niallia circulans F-2 | additional information | - |
? | |
| cellohexaose + H2O | - |
Niallia circulans | additional information | cellotriose + cellobiose + cellotetraose | ? | |
| cellopentaose + H2O | - |
Niallia circulans | additional information | cellobiose + cellotriose | ? | |
| cellopentaose + H2O | - |
Niallia circulans F-2 | additional information | cellobiose + cellotriose | ? | |
| cellotetraose + H2O | - |
Niallia circulans | 2 cellobiose | - |
? | |
| cellotetraose + H2O | - |
Niallia circulans F-2 | 2 cellobiose | - |
? | |
| cellotriose + H2O | no reaction | Niallia circulans | D-glucose + cellobiose | - |
? | |
| cellotriose + H2O | no reaction | Niallia circulans F-2 | D-glucose + cellobiose | - |
? | |
| additional information | hydrolysis of filter paper | Niallia circulans | ? | - |
? | |
| additional information | hydrolysis of filter paper | Niallia circulans F-2 | ? | - |
? | |
| p-nitrophenyl beta-D-cellobioside + H2O | - |
Niallia circulans | glucose + cellobiose + ? | - |
? | |
| xylan + H2O | - |
Niallia circulans | additional information | main products: cellobiose + cellotetraose | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 82000, SDS-PAGE | Niallia circulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Niallia circulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
46 h, stable | Niallia circulans |
| 80 | - |
30 min, 80% loss of activity | Niallia circulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Niallia circulans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | 30°C, 24 h, stable | Niallia circulans |
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