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Literature summary for 3.2.1.4 extracted from
- Molecular cloning and expression of an endo-beta-1,4-D-glucanase I (avicelase I) gene from Bacillus cellulolyticus K-12 and characterization of the recombinant enzyme (1999), Appl. Biochem. Biotechnol., 80, 121-125.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus cellulyticus K-12 |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cellobiose | inhibits hydrolysis of carboxymethylcellulose and avicel | Bacillus cellulyticus K-12 |  |
| Cu2+ | CuCl2 | Bacillus cellulyticus K-12 | |
| Hg2+ | HgCl2 | Bacillus cellulyticus K-12 | |
| PCMB | - |
Bacillus cellulyticus K-12 | |
| SDS | 10 mM, 98% inhibition | Bacillus cellulyticus K-12 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Bacillus cellulyticus K-12 |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates | Bacillus cellulyticus K-12 | |
| Mg2+ | stimulates hydrolysis of avicel and carboxymethylcellulose | Bacillus cellulyticus K-12 | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
1 * 64000, SDS-PAGE | Bacillus cellulyticus K-12 |
| 65000 | - |
gel filtration | Bacillus cellulyticus K-12 |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cellulyticus K-12 | - |
K-12 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus cellulyticus K-12 |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl cellobioside + H2O | - |
Bacillus cellulyticus K-12 | ? | - |
? | |
| avicel + H2O | - |
Bacillus cellulyticus K-12 | ? | cellobiose + cellotetraose | ? | |
| carboxymethylcellulose + H2O | - |
Bacillus cellulyticus K-12 | additional information | - |
? | |
| cellohexaose + H2O | - |
Bacillus cellulyticus K-12 | additional information | cellobiose + cellotetraose | ? | |
| cellopentaose + H2O | - |
Bacillus cellulyticus K-12 | additional information | - |
? | |
| additional information | hydrolysis of filter paper | Bacillus cellulyticus K-12 | ? | - |
? | |
| p-nitrophenyl beta-D-cellobioside + H2O | - |
Bacillus cellulyticus K-12 | glucose + cellobiose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 64000, SDS-PAGE | Bacillus cellulyticus K-12 |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus cellulyticus K-12 |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
48 h, stable | Bacillus cellulyticus K-12 |
| 55 | - |
22% loss of activity after 72 h | Bacillus cellulyticus K-12 |
| 70 | - |
30 min, 80% loss of activity | Bacillus cellulyticus K-12 |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
- |
Bacillus cellulyticus K-12 |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | stable | Bacillus cellulyticus K-12 |
| 4 | 10.5 | stable | Bacillus cellulyticus K-12 |
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