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Literature summary for 3.2.1.4 extracted from

  • Tomme, P.; Gilkes, N.R.; Miller Jr., R.C.; Warren, A.J.; Kilburn, D.G.
    An internal cellulose-binding domain mediates adsorption of an engineered bifunctional xylanase/cellulase (1994), Protein Eng., 7, 117-123.
    View publication on PubMed
Search for more literature for 3.2.1.4

Cloned(Commentary)

Cloned (Comment) Organism
a chimeric xylanase/endoglucanase with an internal cellulase-binding domain by fusing the Bacillus subtilis xyn gene fragment to the 5'-end of the Cellulomonas fimi cenA, overexpression in Escherichia coli Cellulomonas fimi

Protein Variants

Protein Variants Comment Organism
additional information construction of a chimeric xylanase/endoglucanase with an internal cellulase-binding domain by fusing the Bacillus subtilis xyn gene fragment to the 5'-end of the Cellulomonas fimi cenA. The hybrid protein behaves like the parental endoglucanase or xylanase when assayed on a number of soluble and insoluble substrates or xylans Cellulomonas fimi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Cellulomonas fimi
0.2038
-
 2',4'-dinitrophenyl-beta-D-cellobiose endoglucanase A Cellulomonas fimi
0.2085
-
 2',4'-dinitrophenyl-beta-D-cellobiose chimeric xylanase/endoglucanase Cellulomonas fimi

Organism

Organism UniProt Comment Textmining
Cellulomonas fimi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
a chimeric xylanase/endoglucanase with an internal cellulase-binding domain by fusing the Bacillus subtilis xyn gene fragment to the 5'-end of the Cellulomonas fimi cenA, expressed in Escherichia coli Cellulomonas fimi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',4'-dinitrophenyl-beta-D-cellobiose + H2O
-
 Cellulomonas fimi ?
-
 ?
avicel + H2O chimeric xylanase/endoglucanase Cellulomonas fimi ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.358
-
 2',4'-dinitrophenyl-beta-D-cellobiose chimeric xylanase/endoglucanase Cellulomonas fimi
0.397
-
 2',4'-dinitrophenyl-beta-D-cellobiose endoglucanase A Cellulomonas fimi