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Literature summary for 3.2.1.4 extracted from

  • Lew, F.T.; Lewis, L.N.
    Purification and properties of cellulase from Phaseolus vulgaris (1974), Phytochemistry, 13, 1359-1366.
No PubMed abstract available
Search for more literature for 3.2.1.4

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0.1 mM, strong inhibition Phaseolus vulgaris
cellobiose
-
 Phaseolus vulgaris
Hg2+ 1 mM, strong inhibition Phaseolus vulgaris
NEM 70% inhibition of cellulase 4.5 at 10 mM, no effect on cellulase 4.8 Phaseolus vulgaris
PCMB 0.1 mM, 90% loss of activity of cellulase 4.5, 50% loss of activity of cellulase 4.8 Phaseolus vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 gel filtration Phaseolus vulgaris

Organism

Organism UniProt Comment Textmining
Phaseolus vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cellulase 4.5 and cellulase 4.8 Phaseolus vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Phaseolus vulgaris
-

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 43 cellulase 4.8, hydrolysis of carboxymethylcellulose Phaseolus vulgaris
45 60 cellulase 4.5, hydrolysis of carboxymethylcellulose Phaseolus vulgaris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
36
-
 30 min, 50% loss of activity of cellulase 4.8, 25% loss of activity of cellulase 4.5 Phaseolus vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8 5.6 cellulase 4.8 Phaseolus vulgaris
5.7 6.2 cellulase 4.5 Phaseolus vulgaris

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 cellulase 4.5, stable Phaseolus vulgaris
6.5 8 cellulase 4.8, stable Phaseolus vulgaris