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Literature summary for 3.2.1.38 extracted from

  • Giordani, R.; Lafon, L.
    A beta-D-fucosidase from Asclepias curassavica latex (1993), Phytochemistry, 33, 1327-1331.
No PubMed abstract available
Search for more literature for 3.2.1.38

Inhibitors

Inhibitors Comment Organism Structure
2-amino-2-deoxy-D-glucose non-competitive inhibition Asclepias curassavica
Ba2+ strong inhibition at 25 mM Asclepias curassavica
beta-D-fucose competitive inhibitor Asclepias curassavica
beta-D-galactose non-competitive inhibition Asclepias curassavica
beta-D-glucose non-competitive inhibitor Asclepias curassavica
Cu2+ strong inhibition at 25 mM Asclepias curassavica
EDTA inhibition at 25 mM Asclepias curassavica
Fe3+ complete inhibition at 25 mM Asclepias curassavica
Hg2+ complete inhibition at 25 mM Asclepias curassavica
La3+ strong inhibition at 25 mM Asclepias curassavica
Zn2+ strong inhibition at 25 mM Asclepias curassavica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.58
-
 p-nitrophenyl-beta-D-fucoside
-
 Asclepias curassavica

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Asclepias curassavica
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulation at 25 mM Asclepias curassavica
K+ stimulation at 25 mM Asclepias curassavica
Li+ stimulation at 25 mM Asclepias curassavica
Mg2+ stimulation at 25 mM Asclepias curassavica
Mn2+ stimulation at 25 mM Asclepias curassavica
Na+ stimulation at 25 mM Asclepias curassavica
Sr2+ stimulation at 25 mM Asclepias curassavica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000 51000 gel filtration, SDS-PAGE Asclepias curassavica

Organism

Organism UniProt Comment Textmining
Asclepias curassavica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by anion exchange and gel chromatography Asclepias curassavica

Source Tissue

Source Tissue Comment Organism Textmining
latex
-
 Asclepias curassavica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2853
-
-
 Asclepias curassavica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl-2-amino-2-deoxy-D-glucoside + H2O
-
 Asclepias curassavica p-nitrophenol + 2-amino-2-deoxy-D-glucose
-
 ?
p-nitrophenyl-alpha-D-galactoside + H2O
-
 Asclepias curassavica p-nitrophenol + alpha-D-galactose
-
 ?
p-nitrophenyl-beta-D-fucoside + H2O
-
 Asclepias curassavica p-nitrophenol + beta-D-fucose
-
 ?
p-nitrophenyl-beta-D-galactoside + H2O
-
 Asclepias curassavica p-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-glucoside + H2O
-
 Asclepias curassavica p-nitrophenol + beta-D-glucose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 49000-51000, SDS-PAGE, gel filtration Asclepias curassavica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Asclepias curassavica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to, 10% activity at 55°C Asclepias curassavica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Asclepias curassavica