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Literature summary for 3.2.1.38 extracted from

  • Zeng, Y.; Li, Y.; Gu, Y.; Zhang, S.
    Purification and characterization of a strictly specific beta-D-fucosidase from Aspergillus phoenicis (1992), Arch. Biochem. Biophys., 1, 226-230.
    View publication on PubMed
Search for more literature for 3.2.1.38

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activation Aspergillus phoenicis
beta-D-glucose strong activation Aspergillus phoenicis
glycerol activation Aspergillus phoenicis
Salicin activation Aspergillus phoenicis
Serum albumin activation Aspergillus phoenicis

General Stability

General Stability Organism
freezing and thawing inactivates Aspergillus phoenicis

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
-
 Aspergillus phoenicis
4-O-beta-D-glucopyranosyl-D-glucose cellobiose Aspergillus phoenicis
Ag+ strong inhibition Aspergillus phoenicis
beta-D-fucose
-
 Aspergillus phoenicis
D-fucono-gamma-lactone
-
 Aspergillus phoenicis
dicarbonic acid diethyl ester
-
 Aspergillus phoenicis
Hg2+ strong inhibition Aspergillus phoenicis
iodoacetate strong inhibition Aspergillus phoenicis
N-bromosuccinimide
-
 Aspergillus phoenicis
N-ethylmaleimide strong inhibition Aspergillus phoenicis
p-chloromercuribenzoate strong inhibition Aspergillus phoenicis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4
-
 p-nitrophenyl-beta-D-fucoside
-
 Aspergillus phoenicis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 SDS-PAGE, gel filtration Aspergillus phoenicis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-nitrophenyl beta-D-fucoside + H2O Aspergillus phoenicis strictly specific beta-D-fucose + 4-nitrophenol
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
Glycerol 5% v/v required during purification Aspergillus phoenicis

Organism

Organism UniProt Comment Textmining
Aspergillus phoenicis
-
-
-

Oxidation Stability

Oxidation Stability Organism
2-mercaptoethanol, 20 mM required during purification Aspergillus phoenicis

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by aqueous two phase separation, chromatography steps Aspergillus phoenicis

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Aspergillus phoenicis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.6
-
-
 Aspergillus phoenicis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-fucoside + H2O strictly specific Aspergillus phoenicis beta-D-fucose + 4-nitrophenol
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose, weak activity after prolonged incubation Aspergillus phoenicis beta-D-glucose + D-glucose
-
 ?
p-nitrophenyl-beta-D-fucoside + H2O high activity Aspergillus phoenicis p-nitrophenol + beta-D-fucose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 57000, SDS-PAGE, gel filtration Aspergillus phoenicis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Aspergillus phoenicis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 stable below Aspergillus phoenicis
45
-
 inactivation after 30 min Aspergillus phoenicis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Aspergillus phoenicis

pH Range

pH Minimum pH Maximum Comment Organism
5.5 6.5
-
 Aspergillus phoenicis