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Literature summary for 3.2.1.37 extracted from

  • Morana, A.; Mangione, A.; Maurelli, L.; Fiume, I.; Paris, O.; Cannio, R.; Rossi, M.
    Immobilization and characterization of a thermostable beta-xylosidase to generate a reusable biocatalyst (2006), Enzyme Microb. Technol., 39, 1205-1213.
No PubMed abstract available
Search for more literature for 3.2.1.37

Application

Application Comment Organism
synthesis the enzyme immobilized by entrapment into alginate can be used for a continuous production of xylose from xylooligosaccharides at high temperature Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharolobus solfataricus

General Stability

General Stability Organism
immobilized enzyme shows good operational stability retaining 84% of its initial activity and yielding 63% xylose after four cycles of reusing Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97UI4
-
-
Saccharolobus solfataricus P2 Q97UI4
-
-

Storage Stability

Storage Stability Organism
4°C, soluble enzyme, retains 50% of the activity after 2 months Saccharolobus solfataricus
4°C, the enzyme immobilized by entrapment into alginate retains 38% activity after 1 year Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-xylose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Saccharolobus solfataricus P2 4-nitrophenol + beta-D-xylose
-
 ?

Synonyms

Synonyms Comment Organism
SSO3032 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
 assay at Saccharolobus solfataricus
85
-
 soluble enzyme Saccharolobus solfataricus
90
-
 enzyme entrapment in calcium alginate beads Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65 100 65°C: about 60% of maximal activity, 100°C: about 85% of maximal activity, enzyme entrapment in calcium alginate beads Saccharolobus solfataricus
70 90 70°C: about 50% of maximal activity, 90°C: about 25% of maximal activity, soluble enzyme Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 24 h, soluble enzyme and enzyme immobilized by entrapment into alginate are completely stable Saccharolobus solfataricus
90
-
 half life of enzyme immobilized by entrapment into alginate: 21 h. Half-life of soluble enzyme: 10 h Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 soluble enzyme Saccharolobus solfataricus
6.5 7 enzyme entrapped in calcium alginate beads Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 pH 5.5: about 60% of maximal activity, pH 7.5: about 50% of maximal activity, enzyme entrapped in calcium alginate beads Saccharolobus solfataricus
6 7.5 less than 10% of maximal activity at pH 5.5, pH 6.0: maximal activity, pH 7.5: about 60% of maximal activity, soluble enzyme Saccharolobus solfataricus