KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.65 | - |
4-nitrophenyl alpha-L-arabinopyranoside | pH 4.0, 30°C | Talaromyces purpureogenus | |
12 | - |
4-nitrophenyl beta-D-xyloside | pH 4.0, 30°C | Talaromyces purpureogenus | |
12 | - |
4-nitrophenyl alpha-D-xylopyranoside | in 50 mM sodium citrate buffer (pH 4.0), at 30°C | Talaromyces purpureogenus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
gel filtration | Talaromyces purpureogenus |
47305 | - |
1 * 47305, mature protein, calculated from amino acid sequence | Talaromyces purpureogenus |
47305 | - |
1 * 50700, SDS-PAGE, 1 * 47305, calculated | Talaromyces purpureogenus |
50700 | - |
1 * 50700, deduced from SDS-PAGE | Talaromyces purpureogenus |
50700 | - |
1 * 50700, SDS-PAGE, 1 * 47305, calculated | Talaromyces purpureogenus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Talaromyces purpureogenus | C3W4Y2 | bifunctional alpha-L-arabinofuranosidase/xylobiohydrolase | - |
Talaromyces purpureogenus | C3W4Y2 | soft rot fungus | - |
Talaromyces purpureogenus ATCC MYA-38 | C3W4Y2 | bifunctional alpha-L-arabinofuranosidase/xylobiohydrolase | - |
Talaromyces purpureogenus ATCC MYA-38 | C3W4Y2 | soft rot fungus | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Talaromyces purpureogenus |
Purification (Comment) | Organism |
---|---|
- |
Talaromyces purpureogenus |
ammonium sulfate precipitation, DEAE-Sephadex gel filtration, SP Sepharose column chromatography and BioGel P-300 gel filtration | Talaromyces purpureogenus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
89.5 | - |
pH 4.0, 30°C, substrate 4-nitrophenyl alpha-L-arabinopyranoside | Talaromyces purpureogenus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-xylopyranoside + H2O | - |
Talaromyces purpureogenus | 4-nitrophenol + D-xylopyranose | - |
? | |
4-nitrophenyl alpha-D-xylopyranoside + H2O | - |
Talaromyces purpureogenus ATCC MYA-38 | 4-nitrophenol + D-xylopyranose | - |
? | |
4-nitrophenyl alpha-L-arabinopyranoside + H2O | - |
Talaromyces purpureogenus | 4-nitrophenol + alpha-L-arabinopyranose | less than 2% activity with 4-nitrophenyl alpha-L-arabinopyranoside | ? | |
4-nitrophenyl alpha-L-arabinopyranoside + H2O | - |
Talaromyces purpureogenus ATCC MYA-38 | 4-nitrophenol + alpha-L-arabinopyranose | less than 2% activity with 4-nitrophenyl alpha-L-arabinopyranoside | ? | |
4-nitrophenyl beta-D-xyloside + H2O | - |
Talaromyces purpureogenus | 4-nitrophenol + beta-D-xylose | - |
? | |
4-nitrophenyl beta-D-xyloside + H2O | - |
Talaromyces purpureogenus ATCC MYA-38 | 4-nitrophenol + beta-D-xylose | - |
? | |
additional information | less than 2% activity with 4-nitrophenyl alpha-L-arabinopyranoside, 2-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, and 4-nitrophenyl beta-D-galctopyranoside. Only small amounts of xylose are released from oligosaccharides. Smaller oligosaccharides are produced when xylotriose through xylopentaose are used as substrates, suggesting a possible xylobiohydrolase activity. No transglycosylation is detected | Talaromyces purpureogenus | ? | - |
? | |
additional information | less than 2% activity with 4-nitrophenyl alpha-L-arabinopyranoside, 2-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-mannopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-fucopyranoside, 4-nitrophenyl alpha-D-galactopyranoside, and 4-nitrophenyl beta-D-galctopyranoside. Only small amounts of xylose are released from oligosaccharides. Smaller oligosaccharides are produced when xylotriose through xylopentaose are used as substrates, suggesting a possible xylobiohydrolase activity. No transglycosylation is detected | Talaromyces purpureogenus ATCC MYA-38 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47305, mature protein, calculated from amino acid sequence | Talaromyces purpureogenus |
monomer | 1 * 50700, deduced from SDS-PAGE | Talaromyces purpureogenus |
monomer | 1 * 50700, SDS-PAGE, 1 * 47305, calculated | Talaromyces purpureogenus |
Synonyms | Comment | Organism |
---|---|---|
ABF3 | - |
Talaromyces purpureogenus |
ABF3 | bifunctional enzyme having both alpha-L-arabinofuranosidase and xylobiohydrolase functionalities | Talaromyces purpureogenus |
alpha-L-arabinofuranosidase 3 | - |
Talaromyces purpureogenus |
alpha-L-arabinofuranoside arabinofuranohydrolase | - |
Talaromyces purpureogenus |
arabinofuranosidase 3 | bifunctional enzyme having both alpha-L-arabinofuranosidase and xylobiohydrolase functionalities | Talaromyces purpureogenus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Talaromyces purpureogenus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
30 min, 35% loss of activity | Talaromyces purpureogenus |
35 | 50 | the enzyme loses 40% of its activity at 35°C and 90% at 50°C after 30 min of incubation | Talaromyces purpureogenus |
50 | - |
30 min, 90% loss of activity | Talaromyces purpureogenus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.73 | - |
4-nitrophenyl alpha-L-arabinopyranoside | pH 4.0, 30°C | Talaromyces purpureogenus | |
253.3 | - |
4-nitrophenyl alpha-D-xylopyranoside | in 50 mM sodium citrate buffer (pH 4.0), at 30°C | Talaromyces purpureogenus | |
253.3 | - |
4-nitrophenyl beta-D-xyloside | pH 4.0, 30°C | Talaromyces purpureogenus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Talaromyces purpureogenus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.21 | - |
4-nitrophenyl alpha-L-arabinopyranoside | pH 4.0, 30°C | Talaromyces purpureogenus | |
21.1 | - |
4-nitrophenyl beta-D-xyloside | pH 4.0, 30°C | Talaromyces purpureogenus | |
21.7 | - |
4-nitrophenyl alpha-D-xylopyranoside | in 50 mM sodium citrate buffer (pH 4.0), at 30°C | Talaromyces purpureogenus |