Cloned (Comment) | Organism |
---|---|
ecxpression in Escherichia coli | Prevotella ruminicola |
Protein Variants | Comment | Organism |
---|---|---|
D269A | no catalytic activity | Prevotella ruminicola |
D269N | no catalytic activity | Prevotella ruminicola |
E594Q | activity similar to wild-type | Prevotella ruminicola |
E616A | about 5% of wild-type activity | Prevotella ruminicola |
R149L | no catalytic activity | Prevotella ruminicola |
Y237F | less than 10% of wild-type activity | Prevotella ruminicola |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
4-nitrophenyl-beta-D-xylopyranoside | wild-type, pH 5.0, 37°C | Prevotella ruminicola | |
2 | - |
4-nitrophenyl-beta-D-xylopyranoside | mutant E594Q, pH 5.0, 37°C | Prevotella ruminicola | |
3.9 | - |
4-nitrophenyl-beta-D-xylopyranoside | mutant Y237F, pH 5.0, 37°C | Prevotella ruminicola | |
18 | - |
4-nitrophenyl-beta-D-xylopyranoside | mutant E616A, pH 5.0, 37°C | Prevotella ruminicola |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Prevotella ruminicola | D5EY15 | isoform Xyl3A | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | poor substrate | Prevotella ruminicola | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Prevotella ruminicola | 4-nitrophenol + beta-D-xylose | - |
? | |
additional information | no substrate: 4-nitrophenly-beta-D-glucopyranoside. When incubated in combination with bifunctional xylanase-ferulic acid esterase Xyn10D-Fae1A, Xyl3A improves the release of xylose monomers from a hemicellulosic xylan substrate, suggesting that these two enzymes function synergistically to depolymerize xylan | Prevotella ruminicola | ? | - |
? | |
xylobiose + H2O | - |
Prevotella ruminicola | 2 D-xylose | - |
? | |
xylohexaose + H2O | sole products released are xylose monomers | Prevotella ruminicola | 6 D-xylose | - |
? | |
xylopentaose + H2O | sole products released are xylose monomers | Prevotella ruminicola | 5 D-xylose | - |
? | |
xylotetraose + 3 H2O | - |
Prevotella ruminicola | 4 D-xylose | - |
? | |
xylotriose + H2O | - |
Prevotella ruminicola | 3 D-xylose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Xyl3A | - |
Prevotella ruminicola |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
4-nitrophenyl-beta-D-xylopyranoside | mutant Y237F, pH 5.0, 37°C | Prevotella ruminicola | |
3.3 | - |
4-nitrophenyl-beta-D-xylopyranoside | mutant E616A, pH 5.0, 37°C | Prevotella ruminicola | |
9.7 | - |
4-nitrophenyl-beta-D-xylopyranoside | wild-type, pH 5.0, 37°C | Prevotella ruminicola | |
10 | - |
4-nitrophenyl-beta-D-xylopyranoside | mutant E594Q, pH 5.0, 37°C | Prevotella ruminicola |
General Information | Comment | Organism |
---|---|---|
physiological function | when incubated in combination with bifunctional xylanase-ferulic acid esterase Xyn10D-Fae1A, Xyl3A improves the release of xylose monomers from a hemicellulosic xylan substrate, suggesting that these two enzymes function synergistically to depolymerize xylan | Prevotella ruminicola |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
4-nitrophenyl-beta-D-xylopyranoside | mutant E616A, pH 5.0, 37°C | Prevotella ruminicola | |
0.41 | - |
4-nitrophenyl-beta-D-xylopyranoside | mutant Y237F, pH 5.0, 37°C | Prevotella ruminicola | |
5 | - |
4-nitrophenyl-beta-D-xylopyranoside | mutant E594Q, pH 5.0, 37°C | Prevotella ruminicola | |
5.4 | - |
4-nitrophenyl-beta-D-xylopyranoside | wild-type, pH 5.0, 37°C | Prevotella ruminicola |