Cloned (Comment) | Organism |
---|---|
expression in Aspergillus oryzae | Trichoderma reesei |
expression in Aspergillus oryzae | Rasamsonia emersonii |
Crystallization (Comment) | Organism |
---|---|
homology modeling of structure | Trichoderma reesei |
homology modeling of structure | Rasamsonia emersonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 4.0, 40°C | Rasamsonia emersonii | |
0.08 | - |
xylohexaose | pH 4.0, 40°C | Trichoderma reesei | |
0.09 | - |
xylohexaose | pH 4.0, 40°C | Rasamsonia emersonii | |
0.1 | - |
xylopentaose | pH 4.0, 40°C | Trichoderma reesei | |
0.13 | - |
xylopentaose | pH 4.0, 40°C | Rasamsonia emersonii | |
0.15 | - |
xylotetraose | pH 4.0, 40°C | Rasamsonia emersonii | |
0.16 | - |
xylotetraose | pH 4.0, 40°C | Trichoderma reesei | |
0.17 | - |
xylotriose | pH 4.0, 40°C | Rasamsonia emersonii | |
0.19 | - |
xylobiose | pH 4.0, 40°C | Rasamsonia emersonii | |
0.2 | - |
xylotriose | pH 4.0, 40°C | Trichoderma reesei | |
0.4 | - |
xylobiose | pH 4.0, 40°C | Trichoderma reesei | |
0.8 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 4.0, 40°C | Trichoderma reesei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rasamsonia emersonii | - |
- |
- |
Trichoderma reesei | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(Xylbeta(1-4))x + H2O = beta-D-xylopyranose + (Xylbeta(1-4))x-1 | active site residues are D242 and E441 | Rasamsonia emersonii | |
(Xylbeta(1-4))x + H2O = beta-D-xylopyranose + (Xylbeta(1-4))x-1 | active site residues are D264 and E464 | Trichoderma reesei |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1304 | - |
pH 4.0, 60°C | Trichoderma reesei |
3390 | - |
pH 4.0, 60°C | Rasamsonia emersonii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Trichoderma reesei | 4-nitrophenol + beta-D-xylose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Rasamsonia emersonii | 4-nitrophenol + beta-D-xylose | - |
? | |
vinasse + H2O | by-product of ethanol fermentation | Rasamsonia emersonii | D-xylose + ? | 12.1% release of xylose by enzyme alone, combination of enzyme and the multicomponent enzyme product Ultraflo L results in 41.9% release of xylose | ? | |
vinasse + H2O | by-product of ethanol fermentation | Trichoderma reesei | D-xylose + ? | 7.7% release of xylose by enzyme alone, combination of enzyme and the multicomponent enzyme product Ultraflo L results in 40.8% relaese of xylose | ? | |
xylobiose + H2O | - |
Trichoderma reesei | 2 beta-D-xylose | hydrolysis via retention of anomeric configuration | ? | |
xylobiose + H2O | - |
Rasamsonia emersonii | 2 beta-D-xylose | hydrolysis via retention of anomeric configuration | ? | |
xylohexaose + H2O | - |
Trichoderma reesei | beta-D-xylose | - |
? | |
xylohexaose + H2O | - |
Rasamsonia emersonii | beta-D-xylose | - |
? | |
xylopentaose + H2O | - |
Trichoderma reesei | beta-D-xylose | - |
? | |
xylopentaose + H2O | - |
Rasamsonia emersonii | beta-D-xylose | - |
? | |
xylotetraose + H2O | - |
Trichoderma reesei | xylotriose + beta-D-xylose | - |
? | |
xylotetraose + H2O | - |
Rasamsonia emersonii | xylotriose + beta-D-xylose | - |
? | |
xylotriose + 2 H2O | - |
Trichoderma reesei | 3 D-xylose | - |
? | |
xylotriose + 2 H2O | - |
Rasamsonia emersonii | 3 D-xylose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
betaXTE | - |
Rasamsonia emersonii |
betaXTR | - |
Trichoderma reesei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Trichoderma reesei |
60 | - |
- |
Rasamsonia emersonii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable up to | Trichoderma reesei |
60 | - |
stable up to | Rasamsonia emersonii |
70 | - |
30 min, complete inactivation | Trichoderma reesei |
80 | - |
30 min, complete inactivation | Rasamsonia emersonii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
xylobiose | pH 4.0, 40°C | Rasamsonia emersonii | |
0.002 | - |
xylohexaose | pH 4.0, 40°C | Trichoderma reesei | |
0.0027 | - |
xylotetraose | pH 4.0, 40°C | Trichoderma reesei | |
0.0029 | - |
xylotriose | pH 4.0, 40°C | Trichoderma reesei | |
0.0029 | - |
xylohexaose | pH 4.0, 40°C | Rasamsonia emersonii | |
0.003 | - |
xylopentaose | pH 4.0, 40°C | Trichoderma reesei | |
0.0034 | - |
xylopentaose | pH 4.0, 40°C | Rasamsonia emersonii | |
0.0036 | - |
xylotetraose | pH 4.0, 40°C | Rasamsonia emersonii | |
0.0037 | - |
xylotriose | pH 4.0, 40°C | Rasamsonia emersonii | |
0.0044 | - |
xylobiose | pH 4.0, 40°C | Trichoderma reesei | |
0.0147 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 4.0, 40°C | Trichoderma reesei | |
0.017 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 4.0, 40°C | Rasamsonia emersonii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | 3.5 | - |
Trichoderma reesei |
3 | 3.5 | - |
Rasamsonia emersonii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
50% residual activity | Rasamsonia emersonii |
5.5 | - |
50% residual activity | Trichoderma reesei |