KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 4.5, 37°C | Aspergillus awamori | |
6.8 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 4.5, 37°C | Aspergillus awamori | |
55 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 4.5, 37°C | Aspergillus awamori | |
64 | - |
4-nitrophenyl-beta-D-fucopyranoside | pH 4.5, 37°C | Aspergillus awamori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus awamori | - |
- |
- |
Aspergillus awamori X-100 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(Xylbeta(1-4))x + H2O = beta-D-xylopyranose + (Xylbeta(1-4))x-1 | hydrolysis of 4-nitrophenyl-beta-D-xylopyranoside with overall retention of substrate anomeric configuration, double-displacement mechanism. Deglycosylation is the rate-limiting step for the enzymatic hydrolysis | Aspergillus awamori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus awamori | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus awamori X-100 | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Aspergillus awamori | 4-nitrophenol + beta-D-fucose | - |
? | |
4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Aspergillus awamori X-100 | 4-nitrophenol + beta-D-fucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus awamori | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Aspergillus awamori X-100 | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | hydrolysis with overall retention of substrate anomeric configuration | Aspergillus awamori | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | hydrolysis with overall retention of substrate anomeric configuration | Aspergillus awamori X-100 | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
additional information | no substrate: 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside | Aspergillus awamori | ? | - |
? | |
additional information | no substrate: 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside | Aspergillus awamori X-100 | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
4-nitrophenyl-beta-D-fucopyranoside | pH 4.5, 37°C | Aspergillus awamori | |
0.24 | - |
4-nitrophenyl-beta-D-glucopyranoside | pH 4.5, 37°C | Aspergillus awamori | |
17.5 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 4.5, 37°C | Aspergillus awamori | |
31.5 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 4.5, 37°C | Aspergillus awamori |