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Literature summary for 3.2.1.37 extracted from

  • Bravman, T.; Mechaly, A.; Shulami, S.; Belakhov, V.; Baasov, T.; Shoham, G.; Shoham, Y.
    Glutamic acid 160 is the acid-base catalyst of beta-xylosidase from Bacillus stearothermophilus T-6: a family 39 glycoside hydrolase (2001), FEBS Lett., 495, 115-119.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Geobacillus stearothermophilus

Protein Variants

Protein Variants Comment Organism
E160A E160 is the acid-base catalyst Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.016
-
2,5-dinitrophenyl-beta-D-xylopyranoside mutant E160A, pH 7.0, 40°C Geobacillus stearothermophilus
0.025
-
3,4-dinitrophenyl-beta-D-xylopyranoside mutant E160A, pH 7.0, 40°C Geobacillus stearothermophilus
0.18
-
2-nitrophenyl-beta-D-xylopyranoside mutant E160A, pH 7.0, 40°C Geobacillus stearothermophilus
0.27
-
4-nitrophenyl-beta-D-xylopyranoside mutant E160A, pH 7.0, 40°C Geobacillus stearothermophilus
0.8
-
3,4-dinitrophenyl-beta-D-xylopyranoside wild type, pH 7.0, 40°C Geobacillus stearothermophilus
0.95
-
2,5-dinitrophenyl-beta-D-xylopyranoside wild type, pH 7.0, 40°C Geobacillus stearothermophilus
5.2
-
4-nitrophenyl-beta-D-xylopyranoside wild type, pH 7.0, 40°C Geobacillus stearothermophilus
7.1
-
2-nitrophenyl-beta-D-xylopyranoside wild type, pH 7.0, 40°C Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
T-6
-
Geobacillus stearothermophilus T-6
-
T-6
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, expressed in Escherichia coli Geobacillus stearothermophilus

Reaction

Reaction Comment Organism Reaction ID
(Xylbeta(1-4))x + H2O = beta-D-xylopyranose + (Xylbeta(1-4))x-1 Glu160 is the acid-base catalyst, kinetics Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-dinitrophenyl-beta-D-xylopyranoside + H2O
-
Geobacillus stearothermophilus 2,5-dinitrophenol + D-xylopyranose
-
?
2,5-dinitrophenyl-beta-D-xylopyranoside + H2O
-
Geobacillus stearothermophilus T-6 2,5-dinitrophenol + D-xylopyranose
-
?
2-nitrophenyl beta-D-xylopyranoside + H2O
-
Geobacillus stearothermophilus 2-nitrophenol + beta-D-xylopyranose
-
?
2-nitrophenyl beta-D-xylopyranoside + H2O
-
Geobacillus stearothermophilus T-6 2-nitrophenol + beta-D-xylopyranose
-
?
3,4-dinitrophenyl beta-D-xylopyranoside + H2O
-
Geobacillus stearothermophilus 3,4-dinitrophenol + beta-D-xylopyranose
-
?
3,4-dinitrophenyl beta-D-xylopyranoside + H2O
-
Geobacillus stearothermophilus T-6 3,4-dinitrophenol + beta-D-xylopyranose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Geobacillus stearothermophilus 4-nitrophenol + beta-D-xylopyranose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
-
Geobacillus stearothermophilus T-6 4-nitrophenol + beta-D-xylopyranose
-
?