Cloned (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
E160A | E160 is the acid-base catalyst | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
2,5-dinitrophenyl-beta-D-xylopyranoside | mutant E160A, pH 7.0, 40°C | Geobacillus stearothermophilus | |
0.025 | - |
3,4-dinitrophenyl-beta-D-xylopyranoside | mutant E160A, pH 7.0, 40°C | Geobacillus stearothermophilus | |
0.18 | - |
2-nitrophenyl-beta-D-xylopyranoside | mutant E160A, pH 7.0, 40°C | Geobacillus stearothermophilus | |
0.27 | - |
4-nitrophenyl-beta-D-xylopyranoside | mutant E160A, pH 7.0, 40°C | Geobacillus stearothermophilus | |
0.8 | - |
3,4-dinitrophenyl-beta-D-xylopyranoside | wild type, pH 7.0, 40°C | Geobacillus stearothermophilus | |
0.95 | - |
2,5-dinitrophenyl-beta-D-xylopyranoside | wild type, pH 7.0, 40°C | Geobacillus stearothermophilus | |
5.2 | - |
4-nitrophenyl-beta-D-xylopyranoside | wild type, pH 7.0, 40°C | Geobacillus stearothermophilus | |
7.1 | - |
2-nitrophenyl-beta-D-xylopyranoside | wild type, pH 7.0, 40°C | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
T-6 | - |
Geobacillus stearothermophilus T-6 | - |
T-6 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, expressed in Escherichia coli | Geobacillus stearothermophilus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(Xylbeta(1-4))x + H2O = beta-D-xylopyranose + (Xylbeta(1-4))x-1 | Glu160 is the acid-base catalyst, kinetics | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dinitrophenyl-beta-D-xylopyranoside + H2O | - |
Geobacillus stearothermophilus | 2,5-dinitrophenol + D-xylopyranose | - |
? | |
2,5-dinitrophenyl-beta-D-xylopyranoside + H2O | - |
Geobacillus stearothermophilus T-6 | 2,5-dinitrophenol + D-xylopyranose | - |
? | |
2-nitrophenyl beta-D-xylopyranoside + H2O | - |
Geobacillus stearothermophilus | 2-nitrophenol + beta-D-xylopyranose | - |
? | |
2-nitrophenyl beta-D-xylopyranoside + H2O | - |
Geobacillus stearothermophilus T-6 | 2-nitrophenol + beta-D-xylopyranose | - |
? | |
3,4-dinitrophenyl beta-D-xylopyranoside + H2O | - |
Geobacillus stearothermophilus | 3,4-dinitrophenol + beta-D-xylopyranose | - |
? | |
3,4-dinitrophenyl beta-D-xylopyranoside + H2O | - |
Geobacillus stearothermophilus T-6 | 3,4-dinitrophenol + beta-D-xylopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Geobacillus stearothermophilus T-6 | 4-nitrophenol + beta-D-xylopyranose | - |
? |