KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.2 | - |
p-nitrophenyl beta-D-xyloside | - |
Geobacillus stearothermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Geobacillus stearothermophilus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
2 * 75000, SDS-PAGE | Geobacillus stearothermophilus |
150000 | - |
gel filtration, SDS-PAGE | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylan + H2O | Geobacillus stearothermophilus | complete degration of xylan requires synergistic action of two types of enzyme, endo-1,4-xylanase to form xylooligosaccharides and beta-D-xylosidase, which hydrolyses xylooligosaccharides to xylose | beta-D-xylose + ? | - |
? | |
xylo-oligosaccharides + H2O | Geobacillus stearothermophilus | derived from xylan | beta-D-xylose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity by 4step chromatography | Geobacillus stearothermophilus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Geobacillus stearothermophilus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
34.2 | - |
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl beta-D-xyloside + H2O | - |
Geobacillus stearothermophilus | p-nitrophenol + beta-D-xylose | - |
? | |
xylan + H2O | complete degration of xylan requires synergistic action of two types of enzyme, endo-1,4-xylanase to form xylooligosaccharides and beta-D-xylosidase, which hydrolyses xylooligosaccharides to xylose | Geobacillus stearothermophilus | beta-D-xylose + ? | - |
? | |
xylo-oligosaccharides + H2O | derived from xylan | Geobacillus stearothermophilus | beta-D-xylose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 75000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable for 1 h | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | stable within | Geobacillus stearothermophilus |