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Literature summary for 3.2.1.35 extracted from

  • Kaneiwa, T.; Miyazaki, A.; Kogawa, R.; Mizumoto, S.; Sugahara, K.; Yamada, S.
    Identification of amino acid residues required for the substrate specificity of human and mouse chondroitin sulfate hydrolase (conventional hyaluronidase-4) (2012), J. Biol. Chem., 287, 42119-42128.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.35

Cloned(Commentary)

Cloned (Comment) Organism
expressed in COS-7 cells Mus musculus
expressed in COS-7 cells Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 chondroitin sulfate D at pH 4.5 and 37°C Mus musculus
0.66
-
 chondroitin sulfate A at pH 4.5 and 37°C Mus musculus
2.16
-
 chondroitin sulfate C at pH 4.5 and 37°C Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
 Mus musculus
-
placenta
-
 Homo sapiens
-
skeletal muscle
-
 Homo sapiens
-
testis
-
 Mus musculus
-
testis
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chondroitin sulfate A + H2O
-
 Mus musculus ?
-
 ?
chondroitin sulfate A + H2O poor substrate Homo sapiens ?
-
 ?
chondroitin sulfate C + H2O
-
 Mus musculus ?
-
 ?
chondroitin sulfate C + H2O
-
 Homo sapiens ?
-
 ?
chondroitin sulfate D + H2O
-
 Mus musculus ?
-
 ?
chondroitin sulfate D + H2O
-
 Homo sapiens ?
-
 ?
additional information the enzyme depolymerizes various chondroitin sulfate isoforms to a similar extent, suggesting broad substrate specificity Mus musculus ?
-
 ?
additional information the enzyme strongly prefers D-glucuronic acid-(2-O-sulfate)-GalNAc(6-O-sulfate)-containing sequences typical in chondroitin sulfate D Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
chondroitin sulfate hydrolase
-
 Mus musculus
chondroitin sulfate hydrolase
-
 Homo sapiens
HYAL4
-
 Mus musculus
HYAL4
-
 Homo sapiens
hyaluronidase-4
-
 Mus musculus
hyaluronidase-4
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Mus musculus
4.5 5
-
 Homo sapiens

General Information

General Information Comment Organism
physiological function the enzyme play a special role in the testes of adult mice being involved in the maturation of sperm or during fertilization Mus musculus