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Literature summary for 3.2.1.33 extracted from
- The reaction kinetics and the effect of substrate transglycosylation catalyzed by TreX of Sulfolobus solfataricus on glycogen breakdown (2014), J. Bacteriol., 196, 1941-1949.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Eschericha coli MC1061 | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.206 | - |
6-O-alpha-maltotetraosyl-beta-cyclodextrin | pH 5.5, 75°C | Saccharolobus solfataricus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q7LX99 | - |
- |
| Saccharolobus solfataricus P2 | Q7LX99 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-O-alpha-maltotetraosyl-beta-cyclodextrin + H2O | the enzyme catalyzes both hydrolysis of alpha-1,6-glycosidic linkages and transglycosylation at relatively high (above 0.5 mM) substrate concentrations | Saccharolobus solfataricus | ? | - |
? | |
| 6-O-alpha-maltotetraosyl-beta-cyclodextrin + H2O | the enzyme catalyzes both hydrolysis of alpha-1,6-glycosidic linkages and transglycosylation at relatively high (above 0.5 mM) substrate concentrations | Saccharolobus solfataricus P2 | ? | - |
? | |
| maltotetraosyl-alpha-1,6 maltoheptaose + H2O | - |
Saccharolobus solfataricus | ? | - |
? | |
| maltotetraosyl-alpha-1,6 maltoheptaose + H2O | - |
Saccharolobus solfataricus P2 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SSO2094 | locus name | Saccharolobus solfataricus |
| TreX | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
assay at | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2570 | - |
6-O-alpha-maltotetraosyl-beta-cyclodextrin | pH 5.5, 75°C | Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
assay at | Saccharolobus solfataricus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | catalytic efficiency (the kcat/Km ratio) increases as the degree of polymerization of branch chains rises | Saccharolobus solfataricus |
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