Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli MC1061 cells | Nostoc punctiforme |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.51 | - |
maltoundecaose | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme | |
0.62 | - |
maltododecaose | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme | |
0.64 | - |
maltodecaose | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme | |
0.81 | - |
maltononaose | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | Nostoc punctiforme | - |
amylopectin + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc punctiforme | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Nostoc punctiforme |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Nostoc punctiforme | amylopectin + D-glucose | - |
? | |
maltodecaose + H2O | - |
Nostoc punctiforme | ? | - |
? | |
maltodecaosyl-alpha-(1,6)-beta-cyclodextrin + H2O | - |
Nostoc punctiforme | ? | - |
? | |
maltododecaose + H2O | - |
Nostoc punctiforme | ? | - |
? | |
maltoheptaosyl-alpha-(1,6)-beta-cyclodextrin + H2O | - |
Nostoc punctiforme | ? | - |
? | |
maltohexaosyl-alpha-(1,6)-beta-cyclodextrin + H2O | - |
Nostoc punctiforme | ? | - |
? | |
maltononaose + H2O | - |
Nostoc punctiforme | ? | - |
? | |
maltononaosyl-alpha-(1,6)-beta-cyclodextrin + H2O | - |
Nostoc punctiforme | ? | - |
? | |
maltooctaose + H2O | - |
Nostoc punctiforme | ? | - |
? | |
maltooctaosyl-alpha-(1,6)-beta-cyclodextrin + H2O | - |
Nostoc punctiforme | ? | - |
? | |
maltopentaosyl-alpha-(1,6)-beta-cyclodextrin + H2O | - |
Nostoc punctiforme | ? | - |
? | |
maltoundecaose + H2O | - |
Nostoc punctiforme | ? | - |
? | |
maltoundecaosyl-alpha-(1,6)-beta-cyclodextrin + H2O | - |
Nostoc punctiforme | ? | - |
? | |
additional information | NPDE exhibits hydrolysis activity toward both alpha-(1,6)- and alpha-(1,4)-glucosidic linkages, NPDE specifically liberates glucose from the reducing end, the catalytic efficiency of NPDE increases considerably upon using substrates that can occupy at least eight glycone subsites. Side chains shorter than maltooctaose in amylopectin are resistant to hydrolysis by NPDE | Nostoc punctiforme | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycogen debranching enzyme | - |
Nostoc punctiforme |
NPDE | - |
Nostoc punctiforme |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0205 | - |
maltononaose | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme | |
0.045 | - |
maltodecaose | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme | |
0.0452 | - |
maltoundecaose | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme | |
0.0478 | - |
maltododecaose | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
maltooctaose | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme | |
0.0027 | - |
maltopentaosyl-alpha-(1,6)-beta-cyclodextrin | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme | |
0.003 | - |
maltohexaosyl-alpha-(1,6)-beta-cyclodextrin | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme | |
0.0145 | - |
maltoheptaosyl-alpha-(1,6)-beta-cyclodextrin | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme | |
0.025 | - |
maltononaose | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme | |
0.07 | - |
maltodecaose | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme | |
0.077 | - |
maltododecaose | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme | |
0.09 | - |
maltoundecaose | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme | |
0.103 | - |
maltooctaosyl-alpha-(1,6)-beta-cyclodextrin | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme | |
0.117 | - |
maltononaosyl-alpha-(1,6)-beta-cyclodextrin | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme | |
0.173 | - |
maltoundecaosyl-alpha-(1,6)-beta-cyclodextrin | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme | |
0.177 | - |
maltodecaosyl-alpha-(1,6)-beta-cyclodextrin | in 50 mM sodium phosphate buffer (pH 7.5) at 30°C | Nostoc punctiforme |