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Literature summary for 3.2.1.33 extracted from

  • Choi, J.; Lee, H.; Kim, Y.; Park, J.; Woo, E.; Kim, M.; Lee, B.; Park, K.
    Characterization of a novel debranching enzyme from Nostoc punctiforme possessing a high specificity for long branched chains (2009), Biochem. Biophys. Res. Commun., 378, 224-229.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli MC1061 cells Nostoc punctiforme

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.51
-
maltoundecaose in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme
0.62
-
maltododecaose in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme
0.64
-
maltodecaose in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme
0.81
-
maltononaose in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
amylopectin + H2O Nostoc punctiforme
-
amylopectin + D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Nostoc punctiforme
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Nostoc punctiforme

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
Nostoc punctiforme amylopectin + D-glucose
-
?
maltodecaose + H2O
-
Nostoc punctiforme ?
-
?
maltodecaosyl-alpha-(1,6)-beta-cyclodextrin + H2O
-
Nostoc punctiforme ?
-
?
maltododecaose + H2O
-
Nostoc punctiforme ?
-
?
maltoheptaosyl-alpha-(1,6)-beta-cyclodextrin + H2O
-
Nostoc punctiforme ?
-
?
maltohexaosyl-alpha-(1,6)-beta-cyclodextrin + H2O
-
Nostoc punctiforme ?
-
?
maltononaose + H2O
-
Nostoc punctiforme ?
-
?
maltononaosyl-alpha-(1,6)-beta-cyclodextrin + H2O
-
Nostoc punctiforme ?
-
?
maltooctaose + H2O
-
Nostoc punctiforme ?
-
?
maltooctaosyl-alpha-(1,6)-beta-cyclodextrin + H2O
-
Nostoc punctiforme ?
-
?
maltopentaosyl-alpha-(1,6)-beta-cyclodextrin + H2O
-
Nostoc punctiforme ?
-
?
maltoundecaose + H2O
-
Nostoc punctiforme ?
-
?
maltoundecaosyl-alpha-(1,6)-beta-cyclodextrin + H2O
-
Nostoc punctiforme ?
-
?
additional information NPDE exhibits hydrolysis activity toward both alpha-(1,6)- and alpha-(1,4)-glucosidic linkages, NPDE specifically liberates glucose from the reducing end, the catalytic efficiency of NPDE increases considerably upon using substrates that can occupy at least eight glycone subsites. Side chains shorter than maltooctaose in amylopectin are resistant to hydrolysis by NPDE Nostoc punctiforme ?
-
?

Synonyms

Synonyms Comment Organism
glycogen debranching enzyme
-
Nostoc punctiforme
NPDE
-
Nostoc punctiforme

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0205
-
maltononaose in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme
0.045
-
maltodecaose in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme
0.0452
-
maltoundecaose in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme
0.0478
-
maltododecaose in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0027
-
maltooctaose in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme
0.0027
-
maltopentaosyl-alpha-(1,6)-beta-cyclodextrin in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme
0.003
-
maltohexaosyl-alpha-(1,6)-beta-cyclodextrin in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme
0.0145
-
maltoheptaosyl-alpha-(1,6)-beta-cyclodextrin in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme
0.025
-
maltononaose in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme
0.07
-
maltodecaose in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme
0.077
-
maltododecaose in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme
0.09
-
maltoundecaose in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme
0.103
-
maltooctaosyl-alpha-(1,6)-beta-cyclodextrin in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme
0.117
-
maltononaosyl-alpha-(1,6)-beta-cyclodextrin in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme
0.173
-
maltoundecaosyl-alpha-(1,6)-beta-cyclodextrin in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme
0.177
-
maltodecaosyl-alpha-(1,6)-beta-cyclodextrin in 50 mM sodium phosphate buffer (pH 7.5) at 30°C Nostoc punctiforme