Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain MC 1061 | Saccharolobus shibatae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DMSO | 80.8% residual activity at 10% (v/v) | Saccharolobus shibatae | |
ethanol | 83.3% residual activity at 10% (v/v) | Saccharolobus shibatae | |
methanol | 91.6% residual activity at 10% (v/v) | Saccharolobus shibatae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12.23 | - |
6-O-alpha-maltosyl-beta-cyclodextrin | at 70°C in 50 mM sodium acetate buffer (pH 5.5) | Saccharolobus shibatae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 41.7% increase of activity at 5 mM | Saccharolobus shibatae | |
Ca2+ | 27.3% increase of activity at 5 mM | Saccharolobus shibatae | |
Co2+ | 15% increase of activity at 5 mM | Saccharolobus shibatae | |
Fe2+ | 40.6% increase of activity at 5 mM | Saccharolobus shibatae | |
Mg2+ | 17.2% increase of activity at 5 mM | Saccharolobus shibatae | |
Mn2+ | 62.2% increase of activity at 5 mM | Saccharolobus shibatae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus shibatae | - |
strain ATCC 51178 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity chromatography | Saccharolobus shibatae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-O-alpha-maltosyl-beta-cyclodextrin + H2O | - |
Saccharolobus shibatae | maltose + beta-cyclodextrin | - |
? | |
amylopectin + H2O | 100% activity | Saccharolobus shibatae | amylopectin + D-glucose | - |
? | |
amylose + H2O | 16.9% activity compared to amylopectin | Saccharolobus shibatae | ? | - |
? | |
glycogen + H2O | 73.5% activity compared to amylopectin | Saccharolobus shibatae | glycogen + D-glucose | - |
? | |
pullulan + H2O | 36.8% activity compared to amylopectin | Saccharolobus shibatae | ? | - |
? | |
rice starch + H2O | 82.4% activity compared to amylopectin | Saccharolobus shibatae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
amylo-1,6-glucosidase | part of the putative glycogen-debranching enzyme SSGDE | Saccharolobus shibatae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Saccharolobus shibatae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
about 20% of the enzyme activity remains after 60 min of incubation at 100°C | Saccharolobus shibatae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Saccharolobus shibatae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 6.5 | - |
Saccharolobus shibatae |