Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no activating substance | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glycogen particle | protein-glycogen particle from skeletal muscle, glycogen debranching enzyme component of | Oryctolagus cuniculus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
155000 | - |
1 * 155000, glycogen debranching enzyme, sedimentation equilibrium with 6 M guanidinium chloride | Oryctolagus cuniculus |
164000 | - |
glycogen debranching enzyme, sedimentation equilibrium | Oryctolagus cuniculus |
166000 | - |
1 * 166000, glycogen debranching enzyme, SDS-PAGE | Oryctolagus cuniculus |
250000 | - |
glycogen debranching enzyme, low-speed sedimentation equilibrium centrifugation, enzyme has tendency to associate from monomeric form, about 160000 Da, to higher molecular weight species at a protein concentration greater than about 5 mg/ml | Oryctolagus cuniculus |
267000 | 279000 | enzyme has tendency to associate from monomeric form, about 160000 Da, to higher molecular weight species at a protein concentration greater than about 5 mg/ml | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glycogen debranching enzyme | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
muscle | skeletal | Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
4°C, 50 mM glycerol-1-phosphate, 2 mM EDTA, 1 mM dithiothreitol, pH 7.0, 1 month, little or no loss of activity | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycogen + H2O | reverse reaction: incorporation of glucose into glycogen | Oryctolagus cuniculus | glycogen + D-glucose | - |
r | |
glycogen + H2O | slight reversibility | Oryctolagus cuniculus | glycogen + D-glucose | - |
r | |
glycogen phosphorylase-limit dextrin + H2O | - |
Oryctolagus cuniculus | limit dextrin + D-glucose | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 155000, glycogen debranching enzyme, sedimentation equilibrium with 6 M guanidinium chloride | Oryctolagus cuniculus |
monomer | 1 * 166000, glycogen debranching enzyme, SDS-PAGE | Oryctolagus cuniculus |
Synonyms | Comment | Organism |
---|---|---|
glycogen debranching system | EC 3.2.1.33 found in mammals and yeast is in a single polypeptide chain containing two active centres. The other activity is similar to that of EC 2.4.1.25, 4-alpha-glucanotransferase, which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-alpha-glucosidase activity can hydrolyse. Together, these two activities constitute the glycogen debranching system | Oryctolagus cuniculus |