Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus subtilis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
x * 20000, SDS-PAGE, x * 20000-24000, gel filtration | Bacillus subtilis |
340000 | - |
PAGE | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
CCMI 966, enzyme Xyl II | - |
Purification (Comment) | Organism |
---|---|
partial | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
207 | - |
xylan (oat spelts), pH 6.0, 30°C | Bacillus subtilis |
225 | - |
xylan (birchwood), pH 6.0, 30°C | Bacillus subtilis |
226 | - |
laminarin, pH 6.0, 30°C | Bacillus subtilis |
253 | - |
xylan (larchwood), pH 6.0, 30°C | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethylcellulose + H2O | - |
Bacillus subtilis | ? | - |
? | |
laminarin + H2O | - |
Bacillus subtilis | ? | - |
? | |
xylan (birchwood) + H2O | - |
Bacillus subtilis | xylotetraose + ? | xylotetraose is main product of xylan degradation | ? | |
xylan (larchwood) + H2O | - |
Bacillus subtilis | xylotetraose + ? | xylotetraose is main product of xylan degradation | ? | |
xylan (oat spelts) + H2O | - |
Bacillus subtilis | xylotetraose + ? | xylotetraose is main product of xylan degradation | ? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme shows no xylan-binding domain | Bacillus subtilis |
multimer | x * 20000, SDS-PAGE, x * 20000-24000, gel filtration | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
activity increases with temperature, denaturation above | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
180 min, stable | Bacillus subtilis |
50 | - |
92 min, 50% loss of activity | Bacillus subtilis |
60 | - |
20 min, 50% loss of activity | Bacillus subtilis |
70 | - |
8 min, 50% loss of activity | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Bacillus subtilis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus subtilis | isoelectric focusing | - |
7.5 |