Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.3 extracted from

  • Riaz, M.; Rashid, M.; Sawyer, L.; Akhtar, S.; Javed, M.; Nadeem, H.; Wear, M.
    Physiochemical properties and kinetics of glucoamylase produced from deoxy-D-glucose resistant mutant of Aspergillus niger for soluble starch hydrolysis (2012), Food Chem., 130, 24-30.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information thermodynamics and kinetics of starch hydrolysis, overview Aspergillus niger
0.16
-
starch mutant enzyme, pH 5.0, 40°C Aspergillus niger
0.25
-
starch wild-type enzyme, pH 5.0, 40°C Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72063
-
1 * 93000, wild-type and mutant enzyme, SDS-PAGE, 1 * 72876, wild-type enzyme, mass spectrometry, 1 * 72063, mutant enzyme, mass spectrometry Aspergillus niger
72876
-
1 * 93000, wild-type and mutant enzyme, SDS-PAGE, 1 * 72876, wild-type enzyme, mass spectrometry, 1 * 72063, mutant enzyme, mass spectrometry Aspergillus niger
93000
-
1 * 93000, wild-type and mutant enzyme, SDS-PAGE, 1 * 72876, wild-type enzyme, mass spectrometry, 1 * 72063, mutant enzyme, mass spectrometry Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
locally isolated Aspergillus niger, wild-type and deoxy-D-glucose-resistant mutant
-

Purification (Commentary)

Purification (Comment) Organism
glucoamylases from a wild-type and a deoxy-D-glucose-resistant mutant Aspergillus niger to apparent homogeneity by ammonium sulfate fractionation, anion exchange chromatography, and hydrophobic interaction chromatography, the wild-tyype enzyme is purified 25.4fold, the mutant 30.6fold Aspergillus niger

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
216
-
purified wild-type enzyme, pH 5.0, 40°C, substrate starch Aspergillus niger
495.2
-
purified mutant enzyme, pH 5.0, 40°C, substrate starch Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O
-
Aspergillus niger beta-D-glucose + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 93000, wild-type and mutant enzyme, SDS-PAGE, 1 * 72876, wild-type enzyme, mass spectrometry, 1 * 72063, mutant enzyme, mass spectrometry Aspergillus niger

Synonyms

Synonyms Comment Organism
glucoamylase
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
assay at Aspergillus niger

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 70 activity range Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
343
-
starch wild-type enzyme, pH 5.0, 40°C Aspergillus niger
727
-
starch mutant enzyme, pH 5.0, 40°C Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
assay at Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
3 6 activity range Aspergillus niger

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1347
-
starch wild-type enzyme, pH 5.0, 40°C Aspergillus niger
4510
-
starch mutant enzyme, pH 5.0, 40°C Aspergillus niger