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Literature summary for 3.2.1.3 extracted from

  • Kim, J.H.; Kim, H.R.; Lim, M.H.; Ko, H.M.; Chin, J.E.; Lee, H.B.; Kim, I.C.; Bai, S.
    Construction of a direct starch-fermenting industrial strain of Saccharomyces cerevisiae producing glucoamylase, alpha-amylase and debranching enzyme (2010), Biotechnol. Lett., 32, 713-719.
    View publication on PubMed
Search for more literature for 3.2.1.3

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
 SDS-PAGE Aspergillus awamori

Organism

Organism UniProt Comment Textmining
Aspergillus awamori
-
 gene of glucoamylase from Aspergillus awamori integrated into genome of Saccharomyces cerevisiae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 0.12 U/ml, substrate pullulan Aspergillus awamori
additional information
-
 0.51 U/ml, substrate isomaltose Aspergillus awamori
additional information
-
 values between 0.56 to 1.02 U/ml for different transformants Aspergillus awamori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isomaltose + H2O assay at pH 5.5, 40°C Aspergillus awamori ?
-
 ?
pullulan + H2O assay at pH 5.5, 40°C Aspergillus awamori ?
-
 ?

Synonyms

Synonyms Comment Organism
GA1
-
 Aspergillus awamori
glucoamylase
-
 Aspergillus awamori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Aspergillus awamori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Aspergillus awamori