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Literature summary for 3.2.1.3 extracted from
- Overproduction of glucoamylase by a deregulated mutant of a thermophilic mould thermomucor indicae-seudaticae (2008), Appl. Biochem. Biotechnol., 158, 113-125.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | enzyme is mutagenised using nitrous acid and gamma (60Co) irradiation in a sequential manner to isolate deregulated mutants for enhanced production of glucoamylase | Thermomucor indicae-seudaticae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermomucor indicae-seudaticae | - |
CR19 | - |
| Thermomucor indicae-seudaticae CR19 | - |
CR19 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| crude glucoamylase preparation is partially purified by acetone precipitation (80% saturation) and is used in enzyme assays | Thermomucor indicae-seudaticae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclodextrin + H2O | - |
Thermomucor indicae-seudaticae | ? | - |
? |  |
| amylopectin + H2O | - |
Thermomucor indicae-seudaticae | beta-D-glucose + ? | - |
? | |
| amylose + H2O | - |
Thermomucor indicae-seudaticae | beta-D-glucose + ? | - |
? | |
| dextrin + H2O | - |
Thermomucor indicae-seudaticae | ? | - |
? | |
| dextrin + H2O | - |
Thermomucor indicae-seudaticae CR19 | ? | - |
? | |
| maltose + H2O | - |
Thermomucor indicae-seudaticae | ? | - |
? | |
| maltose + H2O | - |
Thermomucor indicae-seudaticae CR19 | ? | - |
? | |
| additional information | displays broad substrate specificity by cleaving alpha-1,4- and alpha-1,6-glycosidic linkages in starch, amylopectin, amylose and pullulan. No activity is observed on alpha-cyclodextrin | Thermomucor indicae-seudaticae | ? | - |
? | |
| additional information | displays broad substrate specificity by cleaving alpha-1,4- and alpha-1,6-glycosidic linkages in starch, amylopectin, amylose and pullulan. No activity is observed on alpha-cyclodextrin | Thermomucor indicae-seudaticae CR19 | ? | - |
? | |
| pullulan + H2O | - |
Thermomucor indicae-seudaticae | ? | - |
? | |
| pullulan + H2O | - |
Thermomucor indicae-seudaticae CR19 | ? | - |
? | |
| starch + H2O | - |
Thermomucor indicae-seudaticae | ? | - |
? | |
| starch + H2O | - |
Thermomucor indicae-seudaticae CR19 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-1,4-D-glucan glucohydrolase | - |
Thermomucor indicae-seudaticae |
| glucoamylase | - |
Thermomucor indicae-seudaticae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
optimum temperature for growth and glucoamylase production is 40°C | Thermomucor indicae-seudaticae |
| 60 | - |
parent and mutant strains are optimally active at | Thermomucor indicae-seudaticae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Thermomucor indicae-seudaticae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
there is no growth and glucoamylase production at pH 10.0 and above and below 3.0 | Thermomucor indicae-seudaticae |
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