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Literature summary for 3.2.1.3 extracted from
- Immobilization of Aspergillus niger glucoamylase onto a polyaniline polymer (2004), Process Biochem., 40, 1155-1159.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| industry | immobilization of the enzyme on polyaniline polymer allows an improved catalytic performance and application of the enzyme in industrial processes | Aspergillus niger |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | immobilization of the enzyme on polyaniline polymer results in improved catalytic performance with decreased temperature optimum, and increased thermal stability and catalytic efficiency with increased Vmax and reduced Km, overview | Aspergillus niger |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.43 | - |
starch | pH 4.2, 60°C, immobilized enzyme | Aspergillus niger |  |
| 4.76 | - |
starch | pH 4.2, 60°C, free enzyme | Aspergillus niger | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Aspergillus niger | soluble starch | starch + beta-D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | immobilization of the enzyme on polyaniline polymer results in improved catalytic performance with decreased temperature optimum, and increased thermal stability and catalytic efficiency, overview | Aspergillus niger | ? | - |
? | |
| starch + H2O | soluble starch | Aspergillus niger | starch + beta-D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucoamylase | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
immobilized enzyme, the enzyme immobilized on polyaniline polymer shows a 10°C decreased temperature optimum | Aspergillus niger |
| 60 | - |
free native enzyme | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme immobilized on polyaniline polymer shows an increased temperature stability compared to the free enzyme | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
assay at | Aspergillus niger |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2 | 8 | 20% of maximal activity at pH 2.0 and 60% at pH 8.0, immobilized enzyme | Aspergillus niger |
| 2 | 7 | 20% of maximal activity at pH 2.0 and pH 7.0, free native enzyme | Aspergillus niger |
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