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Literature summary for 3.2.1.3 extracted from

  • Shiraga, S.; Kawakami, M.; Ueda, M.
    Construction of combinatorial library of starch-binding domain of Rhizopus oryzae glucoamylase and screening of clones with enhanced activity by yeast display method (2004), J. Mol. Catal. B, 28, 229-234.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
construction of combinatorial library of starch-binding domain of the enzyme, expression in Escherichia coli strain DH5alpha, screening of clones with enhanced activity by yeast display method using Saccharomyces cerevisiae strain MT8-1, overview Rhizopus arrhizus

Organism

Organism UniProt Comment Textmining
Rhizopus arrhizus
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O cooked corn starch Rhizopus arrhizus starch + beta-D-glucose
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?

Synonyms

Synonyms Comment Organism
glucoamylase
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Rhizopus arrhizus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
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assay at Rhizopus arrhizus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6
-
assay at Rhizopus arrhizus