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Literature summary for 3.2.1.3 extracted from

  • Morimoto, N.; Hayase, T.; Isono, N.; Tochihara, T.; Watanabe, K.; Ito, H.; Matui, H.
    Cloning, sequencing and heterologous expression of the gene encoding glucoamylase from Clostridium thermoamylolyticum and biochemical characterization of the recombinant enzyme (2004), J. Appl. Glycosci., 51, 33-36.
No PubMed abstract available
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Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, constitutive overexpression of the His-tagged enzyme in Escherichia coli Clostridium thermoamylolyticum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4
-
 maltose pH 4.5, 65°C, recombinant enzyme Clostridium thermoamylolyticum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
77000
-
 x * 77000, recombinant enzyme, SDS-PAGE, x * 79920, amino acid sequence calculation Clostridium thermoamylolyticum
79920
-
 x * 77000, recombinant enzyme, SDS-PAGE, x * 79920, amino acid sequence calculation Clostridium thermoamylolyticum

Organism

Organism UniProt Comment Textmining
Clostridium thermoamylolyticum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme 10fold from Escherichia coli by nickel affinity chromatography Clostridium thermoamylolyticum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8
-
 purified recombinant enzyme Clostridium thermoamylolyticum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltose + H2O
-
 Clostridium thermoamylolyticum beta-D-glucose + D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 77000, recombinant enzyme, SDS-PAGE, x * 79920, amino acid sequence calculation Clostridium thermoamylolyticum

Synonyms

Synonyms Comment Organism
glucoamylase
-
 Clostridium thermoamylolyticum
More the enzyme belongs to the glycoside hydrolase family 15 Clostridium thermoamylolyticum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Clostridium thermoamylolyticum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 80% remaining activity Clostridium thermoamylolyticum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.1
-
 maltose pH 4.5, recombinant enzyme Clostridium thermoamylolyticum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 recombinant enzyme Clostridium thermoamylolyticum

pH Range

pH Minimum pH Maximum Comment Organism
3.9 9 over 80% at pH 3.9 and pH 9.0 Clostridium thermoamylolyticum