Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain AH22, secretion of recombinant enzymes | Saccharomycopsis fibuligera |
Crystallization (Comment) | Organism |
---|---|
purified recombinant nonglycosylated enzyme, 10 mg/ml protein in 50 mM acetate, pH 5.4, with 15% PEG 8000, X-ray diffraction structure determination and analysis at 1.1-1.6 A resolution, modelling | Saccharomycopsis fibuligera |
Protein Variants | Comment | Organism |
---|---|---|
H447A | site-directed mutagenesis, structure analysis compared to the wild-type, crystal structure | Saccharomycopsis fibuligera |
H447A/D450A | site-directed mutagenesis, structure analysis compared to the wild-type, crystal structure | Saccharomycopsis fibuligera |
R15A | site-directed mutagenesis, structure analysis compared to the wild-type, crystal structure | Saccharomycopsis fibuligera |
T462A | site-directed mutagenesis, structure analysis compared to the wild-type, crystal structure | Saccharomycopsis fibuligera |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | enzyme secretion | Saccharomycopsis fibuligera | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Saccharomycopsis fibuligera | - |
starch + beta-D-glucose | - |
? | |
starch + H2O | Saccharomycopsis fibuligera HUT 7212 | - |
starch + beta-D-glucose | - |
? | |
starch + H2O | Saccharomycopsis fibuligera IFO 0111 | - |
starch + beta-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomycopsis fibuligera | P08017 | Glu1 precursor | - |
Saccharomycopsis fibuligera | Q8TFE5 | Glu-1.1 precursor | - |
Saccharomycopsis fibuligera HUT 7212 | P08017 | Glu1 precursor | - |
Saccharomycopsis fibuligera IFO 0111 | Q8TFE5 | Glu-1.1 precursor | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Saccharomycopsis fibuligera |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Saccharomyces cerevisiae strain AH22 medium by gel filtration and ion exchange chromatography to homogeneity | Saccharomycopsis fibuligera |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-1-alpha-D-glucopyranose + beta-D-glucopyranose | active site structure, catalytic residue is Tyr464, catalytic domain and raw starch binding site | Saccharomycopsis fibuligera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Saccharomycopsis fibuligera | starch + beta-D-glucose | - |
? | |
starch + H2O | - |
Saccharomycopsis fibuligera HUT 7212 | starch + beta-D-glucose | - |
? | |
starch + H2O | - |
Saccharomycopsis fibuligera IFO 0111 | starch + beta-D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme consists of a catalytic domain and a starch binding domain connected by an O-glycosylated peptide linker located at the N-terminus, the enzyme contains 7 subsites for substrate binding | Saccharomycopsis fibuligera |
Synonyms | Comment | Organism |
---|---|---|
alpha-1,4-D-glucan glucohydrolase | - |
Saccharomycopsis fibuligera |
Glu-1.1 | - |
Saccharomycopsis fibuligera |
Glu-A | - |
Saccharomycopsis fibuligera |
Glu1 | - |
Saccharomycopsis fibuligera |
Glucan 1,4-alpha-glucosidase | - |
Saccharomycopsis fibuligera |
glucoamylase | - |
Saccharomycopsis fibuligera |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Saccharomycopsis fibuligera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
assay at | Saccharomycopsis fibuligera |