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Literature summary for 3.2.1.3 extracted from
- Purification and properties of glucoamylase from sugar beet cells in suspension culture (1988), Plant Physiol., 88, 172-177.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | 7.8 mg/ml for soluble starch | Beta vulgaris |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | in callus the enzyme is present in the soluble fraction, in suspension culture it is present predominantly in the extracellular fraction | Beta vulgaris | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 83000 | - |
x * 83000, SDS-PAGE | Beta vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Beta vulgaris | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Beta vulgaris |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Beta vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| callus | - |
Beta vulgaris | - |
| cell culture | - |
Beta vulgaris | - |
| root | mature | Beta vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 94.7 | - |
callus | Beta vulgaris |
| 102.2 | - |
suspension culture | Beta vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | soluble | Beta vulgaris | glucose + ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 83000, SDS-PAGE | Beta vulgaris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Beta vulgaris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
10 min, stable | Beta vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.4 | - |
- |
Beta vulgaris |
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Release 2023.1 (January 2023)
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