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Literature summary for 3.2.1.28 extracted from

  • Franco, A.; Soto, T.; Vicente-Soler, J.; Paredes, V.; Madrid, M.; Gacto, M.; Cansado, J.
    A role for calcium in the regulation of neutral trehalase activity in the fission yeast Schizosaccharomyces pombe (2003), Biochem. J., 376, 209-217.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.28

Protein Variants

Protein Variants Comment Organism
D108K mutant enzyme fails to bind Ca2+ or to undergo enzyme activation Schizosaccharomyces pombe
D97L mutant enzyme fails to bind Ca2+ or to undergo enzyme activation Schizosaccharomyces pombe
additional information Ntp1p elutes mainly in an inactive conformation instead of the dimeric or trimeric active form of the enzyme. Activation of the enzyme under different conditions depends upon binding through the Ca2+-binding motif as a prerequiste for correct enzyme oligomerization to its active form Schizosaccharomyces pombe

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ D97 and D108 in the conserved putative Ca2+-binding motif of Ntp1p appear to be responsible for this interaction. Strains expressing Ntp1p variants that are unable to bind Ca2+ partially resemble those devoid of the ntp1+ gene in terms of trehalose hyperaccumulation Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha,alpha-trehalose + H2O Schizosaccharomyces pombe neutral trehalase mobilizes trehalose accumulated by fungal cells as a protective and storage carbohydrate alpha-D-glucopyranose + D-glucose
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 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose + H2O
-
 Schizosaccharomyces pombe alpha-D-glucopyranose + D-glucose
-
 ?
alpha,alpha-trehalose + H2O neutral trehalase mobilizes trehalose accumulated by fungal cells as a protective and storage carbohydrate Schizosaccharomyces pombe alpha-D-glucopyranose + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
Ntp1p
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 Schizosaccharomyces pombe