Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanol | the relative enzyme activity is increasing up to 120% in the presence of 8% (v/v) ethanol at pH 5.5 | Arthrobacter sp. |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli TOP10F' cells and Pichia pastoris strain GS115 | Arthrobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | the enzyme activity is completely inhibited by 5 mM Cu2+ | Arthrobacter sp. | |
Fe2+ | 25% residual activity at 5 mM Fe2+ | Arthrobacter sp. | |
Mn2+ | 11% residual activity at 5 mM Mn2+ | Arthrobacter sp. | |
additional information | the activity of the beta-D-galactosidase is not considerably affected by ditiothreitol, beta-mercaptoethanol, and L-cysteine | Arthrobacter sp. | |
Ni2+ | 38% residual activity at 5 mM Ni2+ | Arthrobacter sp. | |
reduced glutathione | 6% residual activity at 5 mM | Arthrobacter sp. | |
Zn2+ | the enzyme activity is completely inhibited by 5 mM Zn2+ | Arthrobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.62 | - |
2-nitrophenyl-beta-D-galactopyranoside | at 50°C | Arthrobacter sp. | |
3.15 | - |
2-nitrophenyl-beta-D-galactopyranoside | at 40°C | Arthrobacter sp. | |
3.46 | - |
2-nitrophenyl-beta-D-galactopyranoside | at 30°C | Arthrobacter sp. | |
4.86 | - |
2-nitrophenyl-beta-D-galactopyranoside | at 20°C | Arthrobacter sp. | |
5.11 | - |
2-nitrophenyl-beta-D-galactopyranoside | at 55°C | Arthrobacter sp. | |
5.75 | - |
2-nitrophenyl-beta-D-galactopyranoside | at 10°C | Arthrobacter sp. | |
39.73 | - |
lactose | at 50°C | Arthrobacter sp. | |
44.31 | - |
lactose | at 40°C | Arthrobacter sp. | |
53.67 | - |
lactose | at 30°C | Arthrobacter sp. | |
67.82 | - |
lactose | at 20°C | Arthrobacter sp. | |
77.54 | - |
lactose | at 10°C | Arthrobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the activity is not considerably affected by Na+, K+, Mg2+, Co2+, and Ca2+ (5 mM) | Arthrobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
3 * 70000, SDS-PAGE | Arthrobacter sp. |
76140 | - |
subunit, calculated from amino acid sequence | Arthrobacter sp. |
195600 | - |
gel filtration | Arthrobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | C7ASJ5 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and p-aminobenzyl-1-thio-beta-D-galactopyranoside-agarose column chromatography | Arthrobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | the enzyme displays 4times higher level of activity with 4-nitrophenyl beta-D-galactopyranoside than with 2-nitrophenyl beta-D-galactopyranoside | Arthrobacter sp. | 2-nitrophenol + beta-D-galactopyranose | - |
? | |
2-nitrophenyl beta-D-glucopyranoside + H2O | 0.5% of the activity with 2-nitrophenyl beta-D-galactopyranoside | Arthrobacter sp. | 2-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | the enzyme displays 4times higher level of activity with 4-nitrophenyl beta-D-galactopyranoside than with 2-nitrophenyl beta-D-galactopyranoside | Arthrobacter sp. | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | 1.4% of the activity with 2-nitrophenyl beta-D-galactopyranoside | Arthrobacter sp. | 4-nitrophenol + D-glucopyranose | - |
? | |
lactose + H2O | - |
Arthrobacter sp. | D-galactose + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | 3 * 70000, SDS-PAGE | Arthrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Arthrobacter sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
25 | 60% of the maximum activity of the enzyme is determined at 25°C and 15% of the maximum activity is detected at 0°C, the enzyme is active in the range of temperature 4-8°C | Arthrobacter sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.76 | - |
lactose | at 10°C | Arthrobacter sp. | |
2.36 | - |
lactose | at 20°C | Arthrobacter sp. | |
4.81 | - |
lactose | at 30°C | Arthrobacter sp. | |
5.73 | - |
lactose | at 40°C | Arthrobacter sp. | |
6.98 | - |
lactose | at 50°C | Arthrobacter sp. | |
52.4 | - |
2-nitrophenyl-beta-D-galactopyranoside | at 10°C | Arthrobacter sp. | |
71.2 | - |
2-nitrophenyl-beta-D-galactopyranoside | at 55°C | Arthrobacter sp. | |
81 | - |
2-nitrophenyl-beta-D-galactopyranoside | at 20°C | Arthrobacter sp. | |
123.9 | - |
2-nitrophenyl-beta-D-galactopyranoside | at 30°C | Arthrobacter sp. | |
169.9 | - |
2-nitrophenyl-beta-D-galactopyranoside | at 40°C | Arthrobacter sp. | |
212.4 | - |
2-nitrophenyl-beta-D-galactopyranoside | at 50°C | Arthrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Arthrobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | over 90% of the maximum activity in the pH range of 6.5-8.5 | Arthrobacter sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.5 | over 50% of activity at pH 5.5-7.5 at 30°C | Arthrobacter sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Arthrobacter sp. | calculated from amino acid sequence | - |
5.6 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
lactose | at 10°C | Arthrobacter sp. | |
0.035 | - |
lactose | at 20°C | Arthrobacter sp. | |
0.091 | - |
lactose | at 30°C | Arthrobacter sp. | |
0.129 | - |
lactose | at 40°C | Arthrobacter sp. | |
0.176 | - |
lactose | at 50°C | Arthrobacter sp. | |
9.12 | - |
2-nitrophenyl-beta-D-galactopyranoside | at 10°C | Arthrobacter sp. | |
13.93 | - |
2-nitrophenyl-beta-D-galactopyranoside | at 55°C | Arthrobacter sp. | |
16.67 | - |
2-nitrophenyl-beta-D-galactopyranoside | at 20°C | Arthrobacter sp. | |
35.81 | - |
2-nitrophenyl-beta-D-galactopyranoside | at 30°C | Arthrobacter sp. | |
53.92 | - |
2-nitrophenyl-beta-D-galactopyranoside | at 40°C | Arthrobacter sp. | |
81.07 | - |
2-nitrophenyl-beta-D-galactopyranoside | at 50°C | Arthrobacter sp. |