Literature summary for 3.2.1.23 extracted from

Pereira-Rodriguez, A.; Fernandez-Leiro, R.; Gonzalez Siso, M.I.; Cerdan, M.E.; Becerra, M.; Sanz-Aparicio, J.
Crystallization and preliminary X-ray crystallographic analysis of beta-galactosidase from Kluyveromyces lactis (2010), Acta Crystallogr. Sect. F, 66, 297-300.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Saccharomyces cerevisiae BJ3505 cells	Kluyveromyces lactis

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 23-27% (w/v) polyethylene glycol 350, 0.1 M bis-Tris pH 7.5, 0.2 M sodium tartrate	Kluyveromyces lactis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
lactose + H2O	Kluyveromyces lactis	-	D-galactose + D-glucose	-	?

Organism

Organism	UniProt	Comment	Textmining
Kluyveromyces lactis	P00723	-	-

Purification (Commentary)

Purification (Comment)	Organism
anti-FLAG M2 affinity gel filtration	Kluyveromyces lactis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
lactose + H2O	-	Kluyveromyces lactis	D-galactose + D-glucose	-	?

Synonyms

Synonyms	Comment	Organism
beta-D-galactoside galactohydrolase	-	Kluyveromyces lactis
beta-gal	-	Kluyveromyces lactis

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Release 2023.1 (January 2023)