Application | Comment | Organism |
---|---|---|
synthesis | enzyme mutant E184A is a valuable catalyst for the synthesis of metabolically stable analogues of the important glycosidic linkages to the 3 and 4 positions of glucosides and galactosides | Xanthomonas phaseoli pv. manihotis |
Cloned (Comment) | Organism |
---|---|
expression of mutant enzymes in Escherichia coli | Xanthomonas phaseoli pv. manihotis |
Protein Variants | Comment | Organism |
---|---|---|
E184A | site-directed mutagenesis, the mutant functions as an efficient thioglycoligase, which synthesises thiogalactosides with linkages to the 3 and 4 positions of glucosides and galactosides in high yields, the mutant shows reduced galactohydrolase activity compared to the wild-type enzyme | Xanthomonas phaseoli pv. manihotis |
E184Q | site-directed mutagenesis, the mutant does not function as a thioglycoligase, the mutant shows reduced galactohydrolase activity compared to the wild-type enzyme | Xanthomonas phaseoli pv. manihotis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of the thioglycoligase activity of mutant E184A, overview | Xanthomonas phaseoli pv. manihotis | |
0.00008 | - |
3,4-dinitrophenyl beta-D-galactopyranoside | pH 7.0, 30°C, mutant E184A, glycohydrolase activity | Xanthomonas phaseoli pv. manihotis | |
0.002 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 7.0, 30°C, mutant E184A, glycohydrolase activity | Xanthomonas phaseoli pv. manihotis | |
0.05 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 7.0, 30°C, wild-type enzyme, glycohydrolase activity | Xanthomonas phaseoli pv. manihotis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthomonas phaseoli pv. manihotis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant mutant enzymes from Escherichia coli by ammonium sulfate fractionation and hydrophobic interaction chromatography | Xanthomonas phaseoli pv. manihotis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
beta-D-galactopyranosyl-(1-4)-beta-D-galactopyranosyl-(1-6)-beta-D-galactopyranosyl-(1-3)-beta-D-galactopyranose + 3 H2O = 4 beta-D-galactopyranose | Glu184 acts as acid/base catalyst | Xanthomonas phaseoli pv. manihotis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dinitrophenyl beta-D-galactopyranoside + H2O | - |
Xanthomonas phaseoli pv. manihotis | 3,4-dinitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Xanthomonas phaseoli pv. manihotis | 4-nitrophenol + beta-D-galactose | - |
? | |
additional information | the mutant E184A enzyme also acts as thioglycoligase, reaction mechanism, overview | Xanthomonas phaseoli pv. manihotis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BgaX | - |
Xanthomonas phaseoli pv. manihotis |
More | the enzyme belongs to the glycosyl hydrolase family 35 | Xanthomonas phaseoli pv. manihotis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
thioglycoligase assay at | Xanthomonas phaseoli pv. manihotis |
30 | - |
glycohydrolase assay at | Xanthomonas phaseoli pv. manihotis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 7.0, 30°C, mutant E184A, glycohydrolase activity | Xanthomonas phaseoli pv. manihotis | |
2.6 | - |
3,4-dinitrophenyl beta-D-galactopyranoside | pH 7.0, 30°C, mutant E184A, glycohydrolase activity | Xanthomonas phaseoli pv. manihotis | |
2140 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 7.0, 30°C, wild-type enzyme, glycohydrolase activity | Xanthomonas phaseoli pv. manihotis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Xanthomonas phaseoli pv. manihotis |