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Literature summary for 3.2.1.23 extracted from
- Characterization of a thermostable recombinant beta-galactosidase from Thermotoga maritima (2004), J. Appl. Microbiol., 97, 1006-1014.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thermotoga maritima |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | enzyme displays non-Michaelis-Menten reaction kinetics with substrate activation for lactose, which is explained by simultaneous reactions of hydrolysis and transgalactosylation | Thermotoga maritima | |
| 0.33 | - |
o-nitrophenyl-beta-D-galactoside | 80°C | Thermotoga maritima |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | most effective divalent cation for beta-galactosidase activity on both o-nitrophenyl-beta-D-galactoside and lactose | Thermotoga maritima | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 129501 | - |
x * 129501, calculated from sequence | Thermotoga maritima |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + H2O | - |
Thermotoga maritima | D-glucose + D-galactose | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Thermotoga maritima | o-nitrophenol + beta-D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 129501, calculated from sequence | Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
hydrolysis of lactose | Thermotoga maritima |
| 85 | - |
hydrolysis of o-nitrophenyl-beta-D-galactoside | Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
half-life: 16 h | Thermotoga maritima |
| 90 | - |
half-life: 16 min | Thermotoga maritima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
hydrolysis of o-nitrophenyl-beta-D-galactoside and lactose | Thermotoga maritima |
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Release 2023.1 (January 2023)
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