Inhibitors | Comment | Organism | Structure |
---|---|---|---|
o-nitrophenyl-beta-D-galactoside | - |
Aspergillus oryzae | |
o-nitrophenyl-beta-D-galactoside | - |
Escherichia coli | |
o-nitrophenyl-beta-D-galactoside | - |
Kluyveromyces marxianus |
Organic Solvent | Comment | Organism |
---|---|---|
2-methyoxyethyl acetate | 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 70% loss of activity | Escherichia coli |
2-methyoxyethyl acetate | 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 70% loss of activity | Aspergillus oryzae |
Acetone | 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 60% loss of activity | Escherichia coli |
Acetone | 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 75% loss of activity | Aspergillus oryzae |
acetonitrile | 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 60% loss of activity | Escherichia coli |
acetonitrile | 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 70% loss of activity | Aspergillus oryzae |
diethyleneglycol diethyl ether | 37°C, 50% v/v organic solvent-buffer mixture, 5 min,about 60% loss of activity | Escherichia coli |
diethyleneglycol diethyl ether | 37°C, 50% v/v organic solvent-buffer mixture, 5 min,about 90% loss of activity | Aspergillus oryzae |
diglyme | 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 60% loss of activity | Escherichia coli |
diglyme | 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 80% loss of activity | Aspergillus oryzae |
monoglyme | 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 50% loss of activity | Escherichia coli |
monoglyme | 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 75% loss of activity | Aspergillus oryzae |
tetraglyme | 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 40% loss of activity | Escherichia coli |
tetraglyme | 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 75% loss of activity | Aspergillus oryzae |
triethyl phosphate | 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 40% loss of activity | Escherichia coli |
triethyl phosphate | 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 75% loss of activity | Aspergillus oryzae |
triglyme | 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 75% loss of activity | Escherichia coli |
triglyme | 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 80% loss of activity | Aspergillus oryzae |
trimethyl phosphate | 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 60% loss of activity | Escherichia coli |
trimethyl phosphate | 37°C, 50% v/v organic solvent-buffer mixture, 5 min, about 75% loss of activity | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Escherichia coli | - |
- |
- |
Kluyveromyces marxianus | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
beta-galactosidase activity in 80% v/v buffer tetraglyme after 11 h is 9% of the initial activity of the enzyme in 50 mM imidazole/HCl buffer containing 1 mM MgCl2, 145 mM NaCl and 4 mM dithiothreitol at pH 7.0. The recovered activity of the enzyme, measured after dialysis is approximately 45% of the initial activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
o-nitrophenyl-beta-D-galactoside + H2O | no hydrolysis activity is observed in 1,4-dioxane, methylsulphoxide, N,N-dimethylformamide, or pyridine | Escherichia coli | o-nitrophenol + beta-D-galactose | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | no hydrolysis activity is observed in 1,4-dioxane, methylsulphoxide, N,N-dimethylformamide, or pyridine | Kluyveromyces marxianus | o-nitrophenol + beta-D-galactose | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | no hydrolysis activity is observed in 1,4-dioxane, methylsulphoxide, N,N-dimethylformamide, or pyridine | Aspergillus oryzae | o-nitrophenol + beta-D-galactose | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
o-nitrophenyl-beta-D-galactoside | 30°C, in 50 mM imidazole/HCl buffer containing 1 mM MgCl2, 145 mM NaCl and 4 mM dithiothreitol at pH 6.5 | Kluyveromyces marxianus | |
0.28 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, in 50 mM sodium acetate/acetic acid buffer at pH 4.5 | Aspergillus oryzae | |
0.3 | - |
o-nitrophenyl-beta-D-galactoside | 30°C, solvent: 2-methyoxyethyl acetate, 50% v/v organic solvent-buffer mixture | Kluyveromyces marxianus | |
0.3 | - |
o-nitrophenyl-beta-D-galactoside | 30°C, solvent: acetone, 50% v/v organic solvent-buffer mixture | Kluyveromyces marxianus | |
0.3 | - |
o-nitrophenyl-beta-D-galactoside | 30°C, solvent: monoglyme, 50% v/v organic solvent-buffer mixture | Kluyveromyces marxianus | |
0.3 | - |
o-nitrophenyl-beta-D-galactoside | 30°C, solvent: triethyl phosphate, 50% v/v organic solvent-buffer mixture | Kluyveromyces marxianus | |
0.3 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, in 50 mM imidazole/HCl buffer containing 1 mM MgCl2, 145 mM NaCl and 4 mM dithiothreitol at pH 7.0 | Escherichia coli | |
0.35 | - |
o-nitrophenyl-beta-D-galactoside | 30°C, solvent: triglyme, 50% v/v organic solvent-buffer mixture | Kluyveromyces marxianus | |
0.4 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: 2-methyoxyethyl acetate, 50% v/v organic solvent-buffer mixture | Escherichia coli | |
0.42 | - |
o-nitrophenyl-beta-D-galactoside | 30°C, solvent: acetonitrile, 50% v/v organic solvent-buffer mixture | Kluyveromyces marxianus | |
0.58 | - |
o-nitrophenyl-beta-D-galactoside | 30°C, solvent: diglyme, 50% v/v organic solvent-buffer mixture | Kluyveromyces marxianus | |
0.59 | - |
o-nitrophenyl-beta-D-galactoside | 30°C, solvent: trimethyl phosphate, 50% v/v organic solvent-buffer mixture | Kluyveromyces marxianus | |
0.61 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: acetone, 50% v/v organic solvent-buffer mixture | Escherichia coli | |
0.81 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: acetonitrile, 50% v/v organic solvent-buffer mixture | Aspergillus oryzae | |
0.83 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: trimethyl phosphate, 50% v/v organic solvent-buffer mixture | Escherichia coli | |
0.87 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: tetraglyme, 50% v/v organic solvent-buffer mixture | Aspergillus oryzae | |
0.97 | - |
o-nitrophenyl-beta-D-galactoside | 30°C, solvent: diethyleneglycol diethyl ether, 50% v/v organic solvent-buffer mixture | Kluyveromyces marxianus | |
0.99 | - |
o-nitrophenyl-beta-D-galactoside | 30°C, solvent: tetraglyme, 50% v/v organic solvent-buffer mixture | Kluyveromyces marxianus | |
1.04 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: monoglyme, 50% v/v organic solvent-buffer mixture | Escherichia coli | |
1.09 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: diglyme, 50% v/v organic solvent-buffer mixture | Aspergillus oryzae | |
1.21 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: acetonitrile, 50% v/v organic solvent-buffer mixture | Escherichia coli | |
1.38 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: acetone, 50% v/v organic solvent-buffer mixture | Aspergillus oryzae | |
1.4 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: trimethyl phosphate, 50% v/v organic solvent-buffer mixture | Aspergillus oryzae | |
1.44 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: diethyleneglycol diethyl ether, 50% v/v organic solvent-buffer mixture | Escherichia coli | |
1.55 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: diethyleneglycol diethyl ether, 50% v/v organic solvent-buffer mixture | Aspergillus oryzae | |
1.64 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: triglyme, 50% v/v organic solvent-buffer mixture | Aspergillus oryzae | |
1.67 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: tetraglyme, 50% v/v organic solvent-buffer mixture | Escherichia coli | |
1.71 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: diglyme, 50% v/v organic solvent-buffer mixture | Escherichia coli | |
1.76 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: monoglyme, 50% v/v organic solvent-buffer mixture | Aspergillus oryzae | |
1.84 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: triethyl phosphate, 50% v/v organic solvent-buffer mixture | Escherichia coli | |
2.07 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: 2-methyoxyethyl acetate, 50% v/v organic solvent-buffer mixture | Aspergillus oryzae | |
2.92 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: triethyl phosphate, 50% v/v organic solvent-buffer mixture | Aspergillus oryzae | |
3.38 | - |
o-nitrophenyl-beta-D-galactoside | 37°C, solvent: triglyme, 50% v/v organic solvent-buffer mixture | Escherichia coli |