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Literature summary for 3.2.1.23 extracted from
- Purification and characterization of a thermotolerant beta-galactosidase from Thermomyces lanuginosa (1993), Appl. Environ. Microbiol., 61, 1497-1501.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.8 | - |
p-nitrophenyl-beta-D-galactoside | - |
Thermomyces lanuginosus |  |
| 5 | - |
lactulose | - |
Thermomyces lanuginosus | |
| 6.4 | - |
methyl-beta-D-galactoside | - |
Thermomyces lanuginosus | |
| 11.3 | - |
o-nitrophenyl-beta-D-galactoside | - |
Thermomyces lanuginosus | |
| 18.2 | - |
beta-lactose | - |
Thermomyces lanuginosus | |
| 35.9 | - |
phenyl-beta-D-galactoside | - |
Thermomyces lanuginosus | |
| 75.4 | - |
p-nitrophenyl-alpha-L-arabinoside | - |
Thermomyces lanuginosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Thermomyces lanuginosus | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 220000 | - |
- |
Thermomyces lanuginosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Thermomyces lanuginosus | inducible enzyme | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermomyces lanuginosus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Thermomyces lanuginosus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermomyces lanuginosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Thermomyces lanuginosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + H2O | - |
Thermomyces lanuginosus | D-glucose + D-galactose | - |
? | |
| lactulose + H2O | - |
Thermomyces lanuginosus | D-galactose + D-fructose | - |
? | |
| methyl O-beta-galactoside + H2O | - |
Thermomyces lanuginosus | methanol + beta-D-galactose | - |
? | |
| methyl-beta-D-galactopyranoside + H2O | - |
Thermomyces lanuginosus | methanol + beta-D-galactose | - |
? | |
| additional information | inducible enzyme | Thermomyces lanuginosus | ? | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Thermomyces lanuginosus | o-nitrophenol + beta-D-galactose | - |
? | |
| p-nitrophenyl alpha-L-arabinoside + H2O | - |
Thermomyces lanuginosus | p-nitrophenol + alpha-L-arabinose | - |
? | |
| p-nitrophenyl-beta-D-galactoside + H2O | - |
Thermomyces lanuginosus | p-nitrophenol + beta-D-galactose | - |
? | |
| phenyl-beta-D-galactoside + H2O | - |
Thermomyces lanuginosus | phenol + beta-D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | x * 75000-80000, SDS-PAGE | Thermomyces lanuginosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 47 | - |
pH 6.8, 2 h, stable | Thermomyces lanuginosus |
| 50 | - |
191 h, 42% loss of activity | Thermomyces lanuginosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16 | - |
methyl-beta-D-galactoside | - |
Thermomyces lanuginosus | |
| 18 | - |
beta-lactose | - |
Thermomyces lanuginosus | |
| 33 | - |
lactulose | - |
Thermomyces lanuginosus | |
| 65 | - |
phenyl-beta-D-galactoside | - |
Thermomyces lanuginosus | |
| 941 | - |
o-nitrophenyl-beta-D-galactoside | - |
Thermomyces lanuginosus | |
| 1040 | - |
p-nitrophenyl-beta-D-galactoside | - |
Thermomyces lanuginosus | |
| 5000 | - |
p-nitrophenyl-alpha-L-arabinoside | - |
Thermomyces lanuginosus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | 7.2 | - |
Thermomyces lanuginosus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | 37°C, 20 h, stable | Thermomyces lanuginosus |
| 6.8 | - |
47°C, 2 h, stable | Thermomyces lanuginosus |
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