Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.23 extracted from

  • Tsumuraya, Y.; Mochizuki, N.; Hashimoto, Y.; Kovac, P.
    Purification of an exo-beta-(1->3)-D-galactanase of Irpex lacteus (Polyporus tulipiferae) and its action on arabinogalactan-proteins (1990), J. Biol. Chem., 265, 7207-7215.
    View publication on PubMed
Search for more literature for 3.2.1.23

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Irpex lacteus
19.9
-
 beta(1->3)-galactotriose
-
 Irpex lacteus
31.6
-
 beta-(1->3)-galactobiose
-
 Irpex lacteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 gel filtration Irpex lacteus
51000
-
 1 * 51000, denaturing PAGE Irpex lacteus

Organism

Organism UniProt Comment Textmining
Irpex lacteus
-
 i.e. Polyporus tulipiferae
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Irpex lacteus

Purification (Commentary)

Purification (Comment) Organism
-
 Irpex lacteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-(1->3)-D-galactan + H2O
-
 Irpex lacteus beta-D-galactose + ?
-
 ?
beta-1,3-galactobiose + H2O
-
 Irpex lacteus beta-D-galactose
-
 ?
beta-1,3-galactotriose + H2O
-
 Irpex lacteus ?
-
 ?
methyl beta-D-galactopentaoside + H2O
-
 Irpex lacteus ?
-
 ?
methyl beta-D-galactotetraoside + H2O
-
 Irpex lacteus ?
-
 ?
additional information specifically hydrolyzes oligosaccharides and polymers of (1-3)-linked beta-D-galactopyranosyl residues. Hydrolysis of carbohydrate portion of acacia gum and radish arabinogalactan-proteins increases with their decreasing branching. The action pattern of the enzyme includes the capability of bypassing the branching points. The side chains carrying additional D-galactosyl group at the reducing termini are released as neutral (1-6)-linked beta-D-galactooligosaccharides and their acidic derivatives having a 4-O-methyl-beta-D-glucuronosyl residue as the nonreducing end-group Irpex lacteus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 51000, denaturing PAGE Irpex lacteus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
48
-
 pH 4.6, 10 min, stable up to Irpex lacteus
60
-
 pH 4.6, 10 min, complete inactivation Irpex lacteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6
-
 hydrolysis of beta-(1,3)-D-galactan Irpex lacteus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.5 7.5 4°C, 24 h, stable Irpex lacteus