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Literature summary for 3.2.1.23 extracted from
- beta-Galactosidases from Neurospora crassa (1975), Methods Enzymol., 42, 497-503.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
o-nitrophenyl-beta-D-galactoside | beta-D-galactoside galactohydrolase pH 4.2 | Neurospora crassa |  |
| 1.25 | - |
o-nitrophenyl-beta-D-galactoside | beta-D-galactoside galactohydrolase pH 4.5 | Neurospora crassa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 96000 | - |
gel filtration | Neurospora crassa |
| 156000 | - |
beta-D-galactoside galactohydrolase pH 7.5, gel filtration | Neurospora crassa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | beta-D-galactoside galactohydrolase pH 4.2 contains 5% by weight of carbohydrate, 28 mol of covalently bound carbohydrate per mol of protein | Neurospora crassa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| beta-D-galactoside galactohydrolase pH 4.2, beta-D-galactoside galactohydrolase pH 4.5, beta-D-galactoside galactohydrolase pH 7.5 | Neurospora crassa |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 33.25 | - |
beta-D-galactoside galactohydrolase pH 4.2 | Neurospora crassa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, beta-D-galactoside galactohydrolase pH 4.2, stable for 30 days | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Neurospora crassa | o-nitrophenol + beta-D-galactose | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 52 | - |
half-life of beta-D-galactoside galactohydrolase pH 4.2 is 28 min, half-life of the beta-D-galactoside galactohydrolase pH 4.5 is 45 min | Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
beta-D-galactoside galactohydrolase pH 4.2 | Neurospora crassa |
| 4.5 | - |
beta-D-galactoside galactohydrolase pH 4.5 | Neurospora crassa |
| 7.5 | - |
beta-D-galactoside galactohydrolase pH 7.5 | Neurospora crassa |
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Release 2023.1 (January 2023)
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