Crystallization (Comment) | Organism |
---|---|
- |
Paenibacillus macerans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 0.1 mM AgNO3, complete inhibition | Paenibacillus macerans | |
Cu2+ | 0.1 mM, complete inhibition | Paenibacillus macerans | |
D-galactose | - |
Paenibacillus macerans | |
Hg2+ | 0.1 mM HgCl2, complete inhibition | Paenibacillus macerans | |
iodoacetate | - |
Paenibacillus macerans | |
PCMB | - |
Paenibacillus macerans | |
Tris | - |
Paenibacillus macerans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
p-nitrophenyl-beta-D-galactoside | - |
Paenibacillus macerans | |
30 | - |
lactose | - |
Paenibacillus macerans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
4 * 78000, SDS-PAGE | Paenibacillus macerans |
320000 | - |
gel filtration | Paenibacillus macerans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus macerans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Paenibacillus macerans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
279.2 | - |
- |
Paenibacillus macerans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | - |
Paenibacillus macerans | D-glucose + D-galactose | - |
? | |
p-nitrophenyl-beta-D-galactoside + H2O | - |
Paenibacillus macerans | p-nitrophenol + beta-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 78000, SDS-PAGE | Paenibacillus macerans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
in presence of 50 mM glycine, stable below | Paenibacillus macerans |
60 | - |
10 min, complete loss of activity | Paenibacillus macerans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Paenibacillus macerans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 30°C, 2 h, enzyme retains above 95% of its activity | Paenibacillus macerans |