Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1 mM, activates | Thermoanaerobacter sp. | |
Cys | 1 mM, activates | Thermoanaerobacter sp. | |
dithiothreitol | 1 mM, activates | Thermoanaerobacter sp. | |
EDTA | 1 mM, activates | Thermoanaerobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1.5 mM, 45% inhibition | Thermoanaerobacter sp. | |
Co2+ | 1.5 mM, complete inhibition | Thermoanaerobacter sp. | |
Cu2+ | 1.5 mM, complete inhibition | Thermoanaerobacter sp. | |
Fe2+ | 1.5 mM, complete inhibition | Thermoanaerobacter sp. | |
Fe3+ | 1.5 mM, complete inhibition | Thermoanaerobacter sp. | |
Mg2+ | 1.5 mM, 56% inhibition | Thermoanaerobacter sp. | |
Ni2+ | 1.5 mM, complete inhibition | Thermoanaerobacter sp. | |
PCMB | - |
Thermoanaerobacter sp. | |
phenylmethylsulfonyl fluoride | - |
Thermoanaerobacter sp. | |
Zn2+ | 1.5 mM, complete inhibition | Thermoanaerobacter sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.3 | - |
o-nitrophenyl-beta-D-galactoside | - |
Thermoanaerobacter sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Thermoanaerobacter sp. | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 0.1 M, activates | Thermoanaerobacter sp. | |
Li+ | 0.1 M, activates | Thermoanaerobacter sp. | |
Na+ | 0.1 M, activates | Thermoanaerobacter sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
154000 | - |
gel filtration | Thermoanaerobacter sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoanaerobacter sp. | - |
- |
- |
Thermoanaerobacter sp. TP6-B1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermoanaerobacter sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
99 | - |
at 70°C | Thermoanaerobacter sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | - |
Thermoanaerobacter sp. | D-glucose + D-galactose | - |
? | |
lactose + H2O | - |
Thermoanaerobacter sp. TP6-B1 | D-glucose + D-galactose | - |
? | |
methyl O-beta-galactoside + H2O | - |
Thermoanaerobacter sp. | methanol + beta-D-galactose | - |
? | |
methyl O-beta-galactoside + H2O | - |
Thermoanaerobacter sp. TP6-B1 | methanol + beta-D-galactose | - |
? | |
methylthio-beta-D-galactoside + H2O | - |
Thermoanaerobacter sp. | galactose + methanethiol | - |
? | |
methylthio-beta-D-galactoside + H2O | - |
Thermoanaerobacter sp. TP6-B1 | galactose + methanethiol | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Thermoanaerobacter sp. | o-nitrophenol + beta-D-galactose | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Thermoanaerobacter sp. TP6-B1 | o-nitrophenol + beta-D-galactose | - |
? | |
p-nitrophenyl alpha-L-arabinoside + H2O | - |
Thermoanaerobacter sp. | p-nitrophenol + alpha-L-arabinose | - |
? | |
p-nitrophenyl alpha-L-arabinoside + H2O | - |
Thermoanaerobacter sp. TP6-B1 | p-nitrophenol + alpha-L-arabinose | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
half-life: 220 min | Thermoanaerobacter sp. |
75 | - |
half-life: 155 min | Thermoanaerobacter sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
431 | - |
o-nitrophenyl-beta-D-galactoside | - |
Thermoanaerobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
hydrolysis of o-nitrophenyl-beta-D-galactoside | Thermoanaerobacter sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.2 | 7.6 | more than 50% of maximal activity at pH 5.2 and at pH 7.6 | Thermoanaerobacter sp. |