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Literature summary for 3.2.1.23 extracted from
- Properties of immobilized beta-D-galactosidase from Bacillus circulans (1983), Enzyme Microb. Technol., 5, 115-120.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| oligosaccharides | oligosaccharides which are produced as a by-product in the hydrolysis of lactose promote a reversible inactivation of the immobilized enzyme. The inactivation can be reduced by having the reaction in a continuous stirred reactor at a high percent conversion of lactose or by using any immobilized enzyme having a low specific activity | Niallia circulans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 41.7 | - |
lactose | - |
Niallia circulans |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Niallia circulans | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
highest activity for hydrolysis of lactose is obtained with immobilization onto Duolite ES-762 | Niallia circulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + H2O | - |
Niallia circulans | D-glucose + D-galactose | glucose + galactose + considerable amounts of trisaccharides and tetrasaccharides + trace amounts of oligosaccharides | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
hydrolysis of lactose in buffer solution | Niallia circulans |
| 65 | - |
hydrolysis of lactose in skim milk | Niallia circulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
half-life of immobilized enzyme in a continuous stirred reactor: 50 days | Niallia circulans |
| 55 | - |
half-life of immobilized enzyme in a continuous stirred reactor: 15 days | Niallia circulans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
hydrolysis of lactose | Niallia circulans |
| 6.5 | 8.2 | immobilized enzyme | Niallia circulans |
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Release 2023.1 (January 2023)
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