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Literature summary for 3.2.1.23 extracted from
- beta-Galactosidase (1960), The Enzymes, 2nd Ed (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. ), 4, 409-430.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| 4 M urea, irreversible inactivation | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Aspergillus oryzae |  |
| Ag+ | - |
Escherichia coli | |
| Cd2+ | - |
Escherichia coli | |
| Cu2+ | - |
Aspergillus oryzae | |
| Cu2+ | - |
Escherichia coli | |
| Cu2+ | - |
Saccharomyces fragilis | |
| Hg2+ | - |
Aspergillus oryzae | |
| Hg2+ | - |
Escherichia coli | |
| Hg2+ | - |
Felis catus | |
| Hg2+ | - |
Medicago sativa | |
| Hg2+ | - |
Saccharomyces fragilis | |
| Pb2+ | - |
Escherichia coli | |
| PCMB | - |
Escherichia coli | |
| Zn2+ | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Homo sapiens | |
| additional information | - |
additional information | influence of temperature on Km-value of o-nitrophenyl-beta-D-galactoside | Escherichia coli | |
| 0.0513 | - |
p-nitrophenyl-beta-D-galactoside | with 0.05 M NaCl | Escherichia coli | |
| 0.12 | - |
thio-(o-nitrophenyl)-beta-D-galactoside | - |
Escherichia coli | |
| 0.161 | - |
o-nitrophenyl-beta-D-galactoside | with 0.05 M NaCl | Escherichia coli | |
| 0.445 | - |
p-nitrophenyl-beta-D-galactoside | without NaCl | Escherichia coli | |
| 0.95 | - |
o-nitrophenyl-beta-D-galactoside | without NaCl | Escherichia coli | |
| 1.47 | - |
phenyl-beta-D-galactoside | with 0.05 M NaCl | Escherichia coli | |
| 1.9 | - |
alpha-lactose | with 0.05 M NaCl | Escherichia coli | |
| 2.5 | - |
methyl salicylate beta-D-galactoside | - |
Escherichia coli | |
| 3.23 | - |
phenyl-beta-D-galactoside | without NaCl | Escherichia coli | |
| 7.52 | - |
alpha-lactose | without NaCl | Escherichia coli | |
| 8.3 | - |
allolactose | without NaCl | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | - |
Medicago sativa | |
| K+ | - |
Saccharomyces fragilis | |
| K+ | activates hydrolysis of lactose more effectively than Na+ | Escherichia coli | |
| Mg2+ | - |
Medicago sativa | |
| Mg2+ | - |
Saccharomyces fragilis | |
| Mg2+ | strain ML 308 shows absolute requirement, strain ML 309 does not | Escherichia coli | |
| Na+ | - |
Medicago sativa | |
| Na+ | - |
Felis catus | |
| Na+ | activates hydrolysis of o-nitrophenyl-beta-D-galactoside more effectively than K+ | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus flavus | - |
- |
- |
| Aspergillus niger | - |
- |
- |
| Aspergillus oryzae | - |
- |
- |
| Bos taurus | - |
- |
- |
| Canis lupus familiaris | - |
- |
- |
| Capra hircus | - |
- |
- |
| Coffea sp. | - |
coffee | - |
| Escherichia coli | - |
- |
- |
| Escherichia coli K 12 | - |
- |
- |
| Escherichia coli ML 308 | - |
- |
- |
| Escherichia coli ML 309 | - |
- |
- |
| Felis catus | - |
- |
- |
| Helix pomatia | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Klebsiella aerogenes | - |
- |
- |
| Malus sp. | - |
- |
- |
| Medicago sativa | - |
- |
- |
| Neurospora sp. | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
| Ovis aries | - |
- |
- |
| Prunus armeniaca | - |
- |
- |
| Prunus dulcis | - |
almond | - |
| Prunus persica | - |
- |
- |
| Saccharomyces fragilis | - |
- |
- |
| Shigella sonnei | - |
- |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| beta-D-galactopyranosyl-(1-4)-beta-D-galactopyranosyl-(1-6)-beta-D-galactopyranosyl-(1-3)-beta-D-galactopyranose + 3 H2O = 4 beta-D-galactopyranose | mechanism | Escherichia coli |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| intestinal juice | - |
Homo sapiens | - |
| intestine | - |
Homo sapiens | - |
| intestine | mucous membrane | Bos taurus | - |
| mammary gland | - |
Bos taurus | - |
| mammary gland | - |
Felis catus | - |
| mammary gland | - |
Capra hircus | - |
| saliva | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| allolactose + H2O | - |
Escherichia coli | D-galactose + D-glucose | - |
? | |
| allolactose + H2O | - |
Escherichia coli K 12 | D-galactose + D-glucose | - |
? | |
| allolactose + H2O | - |
Escherichia coli ML 308 | D-galactose + D-glucose | - |
? | |
| allolactose + H2O | - |
Escherichia coli ML 309 | D-galactose + D-glucose | - |
? | |
| alpha-lactose + H2O | - |
Escherichia coli | D-galactose + D-glucose | - |
? | |
| lactose + H2O | - |
Homo sapiens | D-glucose + D-galactose | - |
? | |
| lactose + H2O | - |
Aspergillus niger | D-glucose + D-galactose | - |
? | |
| lactose + H2O | - |
Aspergillus oryzae | D-glucose + D-galactose | - |
? | |
| lactose + H2O | alpha-lactose | Escherichia coli | D-glucose + D-galactose | - |
? | |
| lactose + H2O | alpha-lactose | Escherichia coli K 12 | D-glucose + D-galactose | - |
? | |
| lactose + H2O | alpha-lactose | Escherichia coli ML 308 | D-glucose + D-galactose | - |
? | |
| lactose + H2O | alpha-lactose | Escherichia coli ML 309 | D-glucose + D-galactose | - |
? | |
| methyl salicylate beta-D-galactoside + H2O | - |
Escherichia coli | methyl salicylate + beta-D-galactose | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Escherichia coli | o-nitrophenol + beta-D-galactose | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Homo sapiens | o-nitrophenol + beta-D-galactose | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Bos taurus | o-nitrophenol + beta-D-galactose | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Aspergillus niger | o-nitrophenol + beta-D-galactose | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Medicago sativa | o-nitrophenol + beta-D-galactose | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Saccharomyces fragilis | o-nitrophenol + beta-D-galactose | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Aspergillus oryzae | o-nitrophenol + beta-D-galactose | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Escherichia coli K 12 | o-nitrophenol + beta-D-galactose | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Escherichia coli ML 308 | o-nitrophenol + beta-D-galactose | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Escherichia coli ML 309 | o-nitrophenol + beta-D-galactose | - |
? | |
| p-nitrophenyl-beta-D-galactoside + H2O | - |
Escherichia coli | p-nitrophenol + beta-D-galactose | - |
? | |
| p-nitrophenyl-beta-D-galactoside + H2O | - |
Homo sapiens | p-nitrophenol + beta-D-galactose | - |
? | |
| p-nitrophenyl-beta-D-galactoside + H2O | - |
Bos taurus | p-nitrophenol + beta-D-galactose | - |
? | |
| p-nitrophenyl-beta-D-galactoside + H2O | - |
Aspergillus niger | p-nitrophenol + beta-D-galactose | - |
? | |
| p-nitrophenyl-beta-D-galactoside + H2O | - |
Felis catus | p-nitrophenol + beta-D-galactose | - |
? | |
| p-nitrophenyl-beta-D-galactoside + H2O | - |
Escherichia coli K 12 | p-nitrophenol + beta-D-galactose | - |
? | |
| p-nitrophenyl-beta-D-galactoside + H2O | - |
Escherichia coli ML 308 | p-nitrophenol + beta-D-galactose | - |
? | |
| p-nitrophenyl-beta-D-galactoside + H2O | - |
Escherichia coli ML 309 | p-nitrophenol + beta-D-galactose | - |
? | |
| phenyl-beta-D-galactoside + H2O | - |
Escherichia coli | phenol + beta-D-galactose | - |
? | |
| phenyl-beta-D-galactoside + H2O | - |
Escherichia coli K 12 | phenol + beta-D-galactose | - |
? | |
| phenyl-beta-D-galactoside + H2O | - |
Escherichia coli ML 308 | phenol + beta-D-galactose | - |
? | |
| phenyl-beta-D-galactoside + H2O | - |
Escherichia coli ML 309 | phenol + beta-D-galactose | - |
? | |
| thio-(o-nitrophenyl)-beta-D-galactoside + H2O | - |
Escherichia coli | thio-(o-nitrophenol) + beta-D-galactose | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
in presence of 0.05 M NaCl, strain ML 309 | Escherichia coli |
| 7.2 | 7.3 | 30°C, phosphate buffer, strain K-12 | Escherichia coli |
| 7.7 | - |
20°C, 0.05 M Tris-acetate buffer, strain ML 309 | Escherichia coli |
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