Activating Compound | Comment | Organism | Structure |
---|---|---|---|
carbodiimide | 165% activity at 0.4 mM | Diehliomyces microsporus | |
diacetyl | 140% activity at 0.2 mM | Diehliomyces microsporus | |
diethyl dicarbonate | 170% activity at 0.4 mM | Diehliomyces microsporus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 46% residual activity at 2.5 mM | Diehliomyces microsporus | |
Ca2+ | 47% residual activity at 2.5 mM | Diehliomyces microsporus | |
Cd2+ | complete inhibition at 1.25 mM | Diehliomyces microsporus | |
Cu2+ | complete inhibition at 1.25 mM | Diehliomyces microsporus | |
dithiothreitol | 54% residual activity at 0.4 mM | Diehliomyces microsporus | |
Fe2+ | 44% residual activity at 1.25 mM | Diehliomyces microsporus | |
Fe3+ | 6% residual activity at 10 mM | Diehliomyces microsporus | |
Hg2+ | complete inhibition at 1.25 mM | Diehliomyces microsporus | |
K+ | 32% residual activity at 2.5 mM | Diehliomyces microsporus | |
Mg2+ | 63% residual activity at 1.25 mM | Diehliomyces microsporus | |
Mn2+ | 33% residual activity at 5 mM | Diehliomyces microsporus | |
N-bromosuccinimide | complete inhibition at 0.2 mM | Diehliomyces microsporus | |
Pb2+ | 29% residual activity at 5 mM | Diehliomyces microsporus | |
Zn2+ | 24% residual activity at 5 mM | Diehliomyces microsporus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
4-nitrophenyl alpha-D-glucopyranoside | at pH 4.6 and 50°C | Diehliomyces microsporus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
gel filtration | Diehliomyces microsporus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Diehliomyces microsporus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-cellulose column chromatography, Q Sepharose column chromatography, Mono Q column chromatography, and Superdex G-75 gel filtration | Diehliomyces microsporus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.21 | - |
crude extract, at pH 4.6 and 50°C | Diehliomyces microsporus |
11275 | - |
after 1224.1fold purification, at pH 4.6 and 50°C | Diehliomyces microsporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | 100% activity | Diehliomyces microsporus | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
melibiose + H2O | 15.4% activity compared to 4-nitrophenyl alpha-D-glucopyranoside | Diehliomyces microsporus | D-glucose + D-galactose | - |
? | |
additional information | no activity with 2-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-glucuronide, guar gum, and locust bean gum | Diehliomyces microsporus | ? | - |
? | |
raffinose + H2O | 49.95% activity compared to 4-nitrophenyl alpha-D-glucopyranoside | Diehliomyces microsporus | sucrose + D-galactose | - |
? | |
stachyose + H2O | 6.4% activity compared to 4-nitrophenyl alpha-D-glucopyranoside | Diehliomyces microsporus | 2 D-galactose + sucrose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 62000, SDS-PAGE | Diehliomyces microsporus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Diehliomyces microsporus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | about 40% activity at 30°C, about 55% activity at 40°C, about 95% activity at 50°C, 100% activity at 55°C, about 80% activity at 60°C, almost no activity at 70°C | Diehliomyces microsporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Diehliomyces microsporus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6 | the enzyme exhibits 60% and 73% of the maximum activity at pH 4.0 and 6.0, respectively. At pH 3.0 and 7.0, there is hardly any alpha-galactosidase activity remaining | Diehliomyces microsporus |