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Literature summary for 3.2.1.22 extracted from
- Lignocellulose degradation by Phanerochaete chrysosporium Purification and characterization of the main alpha-galactosidase (1999), Biochem. J., 339, 43-53 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-galactose | competitive, product inhibition | Phanerodontia chrysosporium |  |
| galactotetrazole | - |
Phanerodontia chrysosporium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | hydrolysis of alpha-galactosides with poor leaving groups is Michaelian, but that of substrates with good leaving groups exhibits pronounced apparent substrate inhibition, with Kis values similar to Km values | Phanerodontia chrysosporium | |
| 0.03 | - |
alpha-D-galactosyl fluoride | temperature not specified in the publication, pH 5 | Phanerodontia chrysosporium | |
| 0.16 | - |
6-bromo-2-naphthyl alpha-D-galactoside | temperature not specified in the publication, pH 5 | Phanerodontia chrysosporium | |
| 0.2 | - |
4-nitrophenyl alpha-D-galactopyranoside | temperature not specified in the publication, pH 5 | Phanerodontia chrysosporium | |
| 0.22 | - |
1-naphthyl alpha-D-galactoside | temperature not specified in the publication, pH 5 | Phanerodontia chrysosporium | |
| 0.3 | - |
2-nitrophenyl alpha-D-galactopyranoside | temperature not specified in the publication, pH 5 | Phanerodontia chrysosporium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 250000 | - |
gel fitlration | Phanerodontia chrysosporium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phanerodontia chrysosporium | Q9HG00 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | carbohydrate content at least 15% | Phanerodontia chrysosporium |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phanerodontia chrysosporium |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 137 | - |
substrate 4-nitrophenyl alpha-D-galactopyranoside, temperature not specified in the publication, pH 5 | Phanerodontia chrysosporium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 4-5, stable for several months | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl alpha-D-galactoside + H2O | - |
Phanerodontia chrysosporium | 1-naphthol + D-galactose | - |
? | |
| 2,4,6-trinitrophenyl 2-deoxy-2,2-difluoro-alpha-D-galactopyranoside + H2O | - |
Phanerodontia chrysosporium | 2',4',6'-trinitrophenol + 2-deoxy-2,2-difluoro-alpha-D-galactopyranose | - |
? | |
| 2-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Phanerodontia chrysosporium | 2-nitrophenol + alpha-D-galactose | - |
? | |
| 4-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Phanerodontia chrysosporium | 4-nitrophenol + alpha-D-galactose | - |
? | |
| 6-bromo-2-naphthyl alpha-D-galactoside + H2O | - |
Phanerodontia chrysosporium | 6-bromo-2-naphthol + D-galactose | - |
? | |
| alpha-D-galactosyl fluoride + H2O | - |
Phanerodontia chrysosporium | D-galactose + fluoride | - |
? | |
| additional information | enzyme displays a retentive, double-displacement mechanism. No detectable activity on the 4-nitrophenyl alpha-D-glucopyranoside or 4-nitrophenyl alpha-D-mannopyranoside | Phanerodontia chrysosporium | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 60000, SDS-PAGE | Phanerodontia chrysosporium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AgaI | - |
Phanerodontia chrysosporium |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 77 | - |
6-bromo-2-naphthyl alpha-D-galactoside | temperature not specified in the publication, pH 5 | Phanerodontia chrysosporium | |
| 87 | - |
alpha-D-galactosyl fluoride | temperature not specified in the publication, pH 5 | Phanerodontia chrysosporium | |
| 171 | - |
1-naphthyl alpha-D-galactoside | temperature not specified in the publication, pH 5 | Phanerodontia chrysosporium | |
| 272 | - |
4-nitrophenyl alpha-D-galactopyranoside | temperature not specified in the publication, pH 5 | Phanerodontia chrysosporium | |
| 514 | - |
2-nitrophenyl alpha-D-galactopyranoside | temperature not specified in the publication, pH 5 | Phanerodontia chrysosporium | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.8 | - |
- |
Phanerodontia chrysosporium |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.357 | - |
D-galactose | temperature not specified in the publication, pH 5 | Phanerodontia chrysosporium | |
| 7.25 | - |
galactotetrazole | temperature not specified in the publication, pH 5 | Phanerodontia chrysosporium | |
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