Literature summary for 3.2.1.22 extracted from

Fredslund, F.; Hachem, M.A.; Larsen, R.J.; S?rensen, P.G.; Coutinho, P.M.; Lo Leggio, L.; Svensson, B.
Crystal structure of alpha-galactosidase from Lactobacillus acidophilus NCFM: insight into tetramer formation and substrate binding (2011), J. Mol. Biol., 412, 466-480.

Crystallization (Commentary)

Crystallization (Comment)	Organism
the crystal structure of alpha-galactosidase from Lactobacillus acidophilus NCFM (LaMel36A) is determined by single-wavelength anomalous dispersion. In addition, a 1.58-A-resolution crystallographic complex with alpha-D-galactose at substrate binding subsite-1 is determined. LaMel36A has a largeN-terminal twisted beta-sandwich domain, connected by a long alpha-helix to the catalytic (beta/alpha)8-barrel domain, and a C-terminal beta-sheet domain	Lactobacillus acidophilus

Crystallization (Comment)

Organism

the crystal structure of alpha-galactosidase from Lactobacillus acidophilus NCFM (LaMel36A) is determined by single-wavelength anomalous dispersion. In addition, a 1.58-A-resolution crystallographic complex with alpha-D-galactose at substrate binding subsite-1 is determined. LaMel36A has a largeN-terminal twisted beta-sandwich domain, connected by a long alpha-helix to the catalytic (beta/alpha)8-barrel domain, and a C-terminal beta-sheet domain

Lactobacillus acidophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
86000	-	SDS-PAGE	Lactobacillus acidophilus
260000	-	gel filtration	Lactobacillus acidophilus

Organism

Organism	UniProt	Comment	Textmining
Lactobacillus acidophilus	Q7WWP9	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl-alpha-D-galactopyranoside + H2O	-	Lactobacillus acidophilus	4-nitrophenol + alpha-D-galactopyranose	-	?

Subunits

Subunits	Comment	Organism
tetramer	crystal structure, four identical monomers form a tightly packed tetramer where three monomers contribute to the structural integrity of the active site in each monomer	Lactobacillus acidophilus

Synonyms

Synonyms	Comment	Organism
alpha-galactosidase	-	Lactobacillus acidophilus
LaMel36A	-	Lactobacillus acidophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Lactobacillus acidophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	assay at	Lactobacillus acidophilus