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Literature summary for 3.2.1.22 extracted from

  • Gote, M.M.; Khan, M.I.; Gokhale, D.V.; Bastawde, K.B.; Khire, J.M.
    Purification, characterization and substrate specificity of thermostable alpha-galactosidase from Bacillus stearothermophilus (NCIM-5146) (2006), Process Biochem., 41, 1311-1317.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag+ potent inhibitor Geobacillus stearothermophilus
Cu2+ potent inhibitor Geobacillus stearothermophilus
D-galactose
-
Geobacillus stearothermophilus
Hg2+ potent inhibitor Geobacillus stearothermophilus
lactose
-
Geobacillus stearothermophilus
melibiose
-
Geobacillus stearothermophilus
additional information beta-mercaptoethanol (1.0 mM) and urea (1.0 M) do not inhibit the enzyme activity Geobacillus stearothermophilus
PCMB potent inhibitor Geobacillus stearothermophilus
stachyose
-
Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Ba2+, Ca2+, Co2+, Fe3+, K+, Mg2+, Mn2+, Na+, Ni2+, Zn2+ (1 mM) and EDTA (10 mM) have no influence on enzyme activity Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
79900
-
SDS-PAGE Geobacillus stearothermophilus
82950
-
2 * 82950, gel filtration Geobacillus stearothermophilus
165900
-
gel filtration Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-
Geobacillus stearothermophilus NCIM-5146
-
-
-

Purification (Commentary)

Purification (Comment) Organism
phenyl Sepharose CL-4B column chromatography Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.03
-
culture filtrate Geobacillus stearothermophilus
400
-
after 388fold purification Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
melibiose + H2O
-
Geobacillus stearothermophilus D-galactose + D-glucose
-
?
melibiose + H2O
-
Geobacillus stearothermophilus NCIM-5146 D-galactose + D-glucose
-
?
additional information no detectable activity towards methyl-alpha-D-galactopyranoside, locust bean gum, guar gum, and alpha-D-Gal-(1,4)-D-Gal Geobacillus stearothermophilus ?
-
?
additional information no detectable activity towards methyl-alpha-D-galactopyranoside, locust bean gum, guar gum, and alpha-D-Gal-(1,4)-D-Gal Geobacillus stearothermophilus NCIM-5146 ?
-
?
o-nitrophenyl alpha-D-galactopyranoside + H2O
-
Geobacillus stearothermophilus o-nitrophenol + alpha-D-galactose
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Geobacillus stearothermophilus p-nitrophenol + alpha-D-galactose
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Geobacillus stearothermophilus NCIM-5146 p-nitrophenol + alpha-D-galactose
-
?
raffinose + H2O
-
Geobacillus stearothermophilus sucrose + D-galactose
-
?
raffinose + H2O
-
Geobacillus stearothermophilus NCIM-5146 sucrose + D-galactose
-
?
stachyose + 2 H2O
-
Geobacillus stearothermophilus 2 alpha-D-galactose + sucrose
-
?
stachyose + 2 H2O
-
Geobacillus stearothermophilus NCIM-5146 2 alpha-D-galactose + sucrose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 82950, gel filtration Geobacillus stearothermophilus

Synonyms

Synonyms Comment Organism
Alpha-D-galactoside galactohydrolase
-
Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
half-life of inactivation at 70°C is 30 min Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
3 9
-
Geobacillus stearothermophilus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
14.5
-
lactose
-
Geobacillus stearothermophilus
16.25
-
D-galactose
-
Geobacillus stearothermophilus
16.25
-
melibiose
-
Geobacillus stearothermophilus
33
-
stachyose
-
Geobacillus stearothermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Geobacillus stearothermophilus isoelectric focusing
-
4.9