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Literature summary for 3.2.1.22 extracted from

  • Cao, Y.; Yang, P.; Shi, P.; Wang, Y.; Luo, H.; Meng, K.; Zhang, Z.; Wu, N.; Yao, B.; Fan, Y.
    Purification and characterization of a novel protease-resistant alpha-galactosidase from Rhizopus sp. F78 ACCC 30795 (2007), Enzyme Microb. Technol., 41, 835-841.
No PubMed abstract available
Search for more literature for 3.2.1.22

Inhibitors

Inhibitors Comment Organism Structure
Ag+ no activity at 1 mM Rhizopus sp.
Al3+ 66.7% activity at 1 mM Rhizopus sp.
Ca2+ 82.2% activity at 1 mM Rhizopus sp.
Co2+ 96.6% activity at 1 mM Rhizopus sp.
Cr3+ 63.9% activity at 1 mM Rhizopus sp.
Cu2+ 78.1% activity at 1 mM Rhizopus sp.
EDTA 98.1% activity at 1 mM Rhizopus sp.
Fe2+ 75.1% activity at 1 mM Rhizopus sp.
Hg2+ no activity at 1 mM Rhizopus sp.
Li+ 96.7% activity at 1 mM Rhizopus sp.
Mg2+ 98.2% activity at 1 mM Rhizopus sp.
Mn2+ 49.5% activity at 1 mM Rhizopus sp.
additional information not inhibited by Na+ and Zn2+ Rhizopus sp.
Ni(2+) 92.7% activity at 1 mM Rhizopus sp.
SDS no activity at 1 mM Rhizopus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.9
-
 p-nitrophenyl alpha-D-galactopyranoside in 0.1 M McIlvaine buffer (pH 4.8) at 37°C Rhizopus sp.
33
-
 melibiose in 0.1 M McIlvaine buffer (pH 4.8) at 37°C Rhizopus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
K+ 9.5% increased activity at 1 mM Rhizopus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
 SDS-PAGE Rhizopus sp.

Organism

Organism UniProt Comment Textmining
Rhizopus sp.
-
 18S rRNA sequence; ACCC 30795
-
Rhizopus sp. F78
-
 18S rRNA sequence; ACCC 30795
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, HiTrap Q Sepharose XL column chromatography, and Sephacryl S-200 HR gel filtration Rhizopus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2
-
 crude filtrate Rhizopus sp.
74.6
-
 after 60.4fold purification Rhizopus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
melibiose + H2O
-
 Rhizopus sp. D-galactose + D-glucose
-
 ?
melibiose + H2O
-
 Rhizopus sp. F78 D-galactose + D-glucose
-
 ?
additional information no detectable activity towards guar gum Rhizopus sp. ?
-
 ?
additional information no detectable activity towards guar gum Rhizopus sp. F78 ?
-
 ?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Rhizopus sp. p-nitrophenol + alpha-D-galactose
-
 ?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Rhizopus sp. F78 p-nitrophenol + alpha-D-galactose
-
 ?
raffinose + H2O
-
 Rhizopus sp. D-galactose + sucrose
-
 ?
raffinose + H2O
-
 Rhizopus sp. F78 D-galactose + sucrose
-
 ?
stachyose + 2 H2O
-
 Rhizopus sp. 2 alpha-D-galactose + sucrose
-
 ?
stachyose + 2 H2O
-
 Rhizopus sp. F78 2 alpha-D-galactose + sucrose
-
 ?

Synonyms

Synonyms Comment Organism
Aga-F78
-
 Rhizopus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Rhizopus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 activity is 80% of maximum between 40°C and 55°C, the enzyme retains less than 25% of its activity at above 60°C and is stable at 50°C, no activity is lost after 30 min incubation, after 10, 20 and 30 min incubation at 60°C, 38%, 18% and 7% of activity remains, respectively Rhizopus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
 Rhizopus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 10 activity is not detected when the enzyme is reacted in buffers under pH 3.0 or above pH 10.0, activity remains above 80% between pH 4.6-5.2, the enzyme retains more than 80% of its activity between pH 5.0 and 10.0 Rhizopus sp.