Application | Comment | Organism |
---|---|---|
molecular biology | alpha-galactosidase is used as a simple yet powerful reporter enzyme system in Saccharopolyspora erythraea. This reporter sytem has the distinct advantage of quantitatively measuring the level of alpha-galactosidase activity quickly and easily from culture supernatant | Saccharopolyspora erythraea |
Cloned (Comment) | Organism |
---|---|
- |
Saccharopolyspora erythraea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.65 | - |
p-nitrophenyl-alpha-D-galactoside | pH 6, 36°C | Saccharopolyspora erythraea | |
0.65 | - |
4-nitrophenyl-alpha-D-galactopyranoside | pH 6.0, 36°C | Saccharopolyspora erythraea | |
1.4 | - |
melibiose | pH 6, 36°C | Saccharopolyspora erythraea | |
1.4 | - |
melibiose | pH 6.0, 36°C | Saccharopolyspora erythraea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Saccharopolyspora erythraea | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | 34000 | gel filtration | Saccharopolyspora erythraea |
45000 | - |
1 * 45000, SDS-PAGE | Saccharopolyspora erythraea |
45000 | - |
x * 45000, SDS-PAGE | Saccharopolyspora erythraea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharopolyspora erythraea | Q9Z4N5 | - |
- |
Saccharopolyspora erythraea | Q9Z4N5 | alpha-galactosidase precursor | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharopolyspora erythraea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Saccharopolyspora erythraea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-alpha-D-galactopyranoside + H2O | - |
Saccharopolyspora erythraea | 4-nitrophenol + D-galactopyranose | - |
? | |
melibiose + H2O | - |
Saccharopolyspora erythraea | D-galactose + D-glucose | - |
? | |
p-nitrophenyl-alpha-D-galactoside + H2O | - |
Saccharopolyspora erythraea | p-nitrophenol + alpha-D-galactose | - |
? | |
raffinose + H2O | - |
Saccharopolyspora erythraea | sucrose + D-galactose | - |
? | |
stachyose + H2O | - |
Saccharopolyspora erythraea | ? | - |
? | |
stachyose + H2O | - |
Saccharopolyspora erythraea | raffinose + D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Saccharopolyspora erythraea |
monomer | 1 * 45000, SDS-PAGE | Saccharopolyspora erythraea |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
10 min, 15% loss of activity | Saccharopolyspora erythraea |
65 | - |
10 min, 85% residual activity | Saccharopolyspora erythraea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.1 | - |
- |
Saccharopolyspora erythraea |