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Literature summary for 3.2.1.22 extracted from

  • Bryant, R.J.; Rao, D.R.
    Purification and characterization of alpha-galactosidase from peanuts (2001), J. Food Biochem., 25, 139-156.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Arachis hypogaea
Cu2+
-
Arachis hypogaea
D-galactose competitive with p-nitrophenyl alpha-D-galactopyranoside Arachis hypogaea
Hg2+
-
Arachis hypogaea
raffinose competitive with p-nitrophenyl alpha-D-galactopyranoside Arachis hypogaea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.221
-
p-nitrophenyl alpha-D-galactopyranoside
-
Arachis hypogaea
80
-
raffinose
-
Arachis hypogaea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
x * 30000, SDS-PAGE Arachis hypogaea

Organism

Organism UniProt Comment Textmining
Arachis hypogaea
-
type Runner and type Spanish
-

Purification (Commentary)

Purification (Comment) Organism
-
Arachis hypogaea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Arachis hypogaea p-nitrophenol + alpha-D-galactose
-
?
raffinose + H2O
-
Arachis hypogaea sucrose + alpha-D-galactose
-
?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Arachis hypogaea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Arachis hypogaea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Arachis hypogaea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
25.4
-
raffinose competitive with p-nitrophenyl alpha-D-galactopyranoside Arachis hypogaea
189
-
D-galactose competitive with p-nitrophenyl alpha-D-galactopyranoside Arachis hypogaea

pI Value

Organism Comment pI Value Maximum pI Value
Arachis hypogaea isoelectric focusing
-
4.9