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Literature summary for 3.2.1.22 extracted from
- High-yield production and characterization of alpha-galactosidase from Bifidobacterium breve grown on raffinose (2000), Biotechnol. Lett., 22, 747-751.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM, strong inhibition | Bifidobacterium breve |  |
| Hg2+ | 1 mM, strong inhibition | Bifidobacterium breve | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.66 | - |
raffinose | pH 5.5, 37°C | Bifidobacterium breve | |
| 2.14 | - |
melibiose | pH 5.5, 37°C | Bifidobacterium breve | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Bifidobacterium breve | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
2 * 80000, SDS-PAGE | Bifidobacterium breve |
| 160000 | - |
non-denaturing PAGE | Bifidobacterium breve |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Bifidobacterium breve | raffinose induces about twice the activity than melibiose | ? | - |
? | |
| additional information | Bifidobacterium breve 203 | raffinose induces about twice the activity than melibiose | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium breve | - |
- |
- |
| Bifidobacterium breve 203 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bifidobacterium breve |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture condition:raffinose-grown cell | - |
Bifidobacterium breve | - |
| additional information | cell grown on raffinose | Bifidobacterium breve | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 215 | - |
- |
Bifidobacterium breve |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| melibiose + H2O | - |
Bifidobacterium breve | alpha-D-galactose + D-glucose | - |
? | |
| melibiose + H2O | - |
Bifidobacterium breve 203 | alpha-D-galactose + D-glucose | - |
? | |
| additional information | raffinose induces about twice the activity than melibiose | Bifidobacterium breve | ? | - |
? | |
| additional information | raffinose induces about twice the activity than melibiose | Bifidobacterium breve 203 | ? | - |
? | |
| p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Bifidobacterium breve | p-nitrophenol + alpha-D-galactose | - |
? | |
| p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Bifidobacterium breve 203 | p-nitrophenol + alpha-D-galactose | - |
? | |
| raffinose + H2O | - |
Bifidobacterium breve | sucrose + alpha-D-galactose | - |
? | |
| raffinose + H2O | - |
Bifidobacterium breve 203 | sucrose + alpha-D-galactose | - |
? | |
| stachyose + 2 H2O | - |
Bifidobacterium breve | 2 alpha-D-galactose + sucrose | - |
? | |
| stachyose + 2 H2O | - |
Bifidobacterium breve 203 | 2 alpha-D-galactose + sucrose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 80000, SDS-PAGE | Bifidobacterium breve |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6.5 | - |
Bifidobacterium breve |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 12 | 4°C, overnight, stable | Bifidobacterium breve |
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