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Literature summary for 3.2.1.22 extracted from
- Purification and characterization of Phaseolus vulgaris alpha-D-galactosidasr isozymes (1994), Biochem. Mol. Biol. Int., 34, 1055-1062.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.75 | - |
4-nitrophenyl alpha-D-galactopyranoside | - |
Phaseolus vulgaris |  |
| 1.1 | - |
3-nitrophenyl alpha-D-galactopyranoside | - |
Phaseolus vulgaris | |
| 2.27 | - |
2-nitrophenyl alpha-D-galactopyranoside | - |
Phaseolus vulgaris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 149300 | - |
gel filtration | Phaseolus vulgaris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Phaseolus vulgaris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°c, 10 mM sodium citrate, 0.01% NaN3, pH 6.5, one year | Phaseolus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Phaseolus vulgaris | 2-nitrophenol + alpha-D-galactopyranose | - |
? | |
| 3-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Phaseolus vulgaris | 3-nitrophenol + alpha-D-galactopyranose | - |
? | |
| 4-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Phaseolus vulgaris | 4-nitrophenol + alpha-D-galactopyranose | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.238 | - |
2-nitrophenyl alpha-D-galactopyranoside | - |
Phaseolus vulgaris | |
| 0.822 | - |
3-nitrophenyl alpha-D-galactopyranoside | - |
Phaseolus vulgaris | |
| 3.61 | - |
4-nitrophenyl alpha-D-galactopyranoside | - |
Phaseolus vulgaris | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
- |
Phaseolus vulgaris |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.4 | 9 | at 4°C | Phaseolus vulgaris |
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Release 2023.1 (January 2023)
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