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Literature summary for 3.2.1.217 extracted from
- Biochemical characterization of Bombyx mori alpha-N-acetylgalactosaminidase belonging to the glycoside hydrolase family 31 (2021), Insect Mol. Biol., 30, 367-378 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| full-length enzyme and its truncated mutants are expressed in Escherichia coli BL21(DE3) cells | Bombyx mori |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.61 | - |
4-nitrophenyl alpha-N-acetyl-D-galactosaminide | at pH 7.0 and 30°C | Bombyx mori |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 787000 | - |
maltose-binding protein-tagged enzyme, gel filtration | Bombyx mori |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bombyx mori | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| amylose resin column chromatography and Ni-NTA agarose column chromatography | Bombyx mori |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epidermis | - |
Bombyx mori | - |
| fat body | - |
Bombyx mori | - |
| head | lowest expression | Bombyx mori | - |
| larva | - |
Bombyx mori | - |
| malpighian tubule | - |
Bombyx mori | - |
| midgut | - |
Bombyx mori | - |
| ovary | - |
Bombyx mori | - |
| silk gland | - |
Bombyx mori | - |
| testis | highest expression | Bombyx mori | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-N-acetyl-D-galactosaminide + H2O | - |
Bombyx mori | 4-nitrophenol + N-acetyl-D-galactosamine | - |
? |  |
| additional information | no activity with GlcNAc-beta-1,3-GalNAcalpha-pNP | Bombyx mori | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homohexamer | 6 * 166000, maltose-binding protein-tagged enzyme, SDS-PAGE | Bombyx mori |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exo-acting alphaGalNAcase | - |
Bombyx mori |
| Nag31 | - |
Bombyx mori |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 50 | - |
Bombyx mori |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
the enzyme remains stable up to 45°C after 30 min of incubation | Bombyx mori |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.23 | - |
4-nitrophenyl alpha-N-acetyl-D-galactosaminide | at pH 7.0 and 30°C | Bombyx mori |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | - |
Bombyx mori |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | the enzyme is stable (more than 90% residual activity) at pH values ranging from 4.0 to 10.0 at 4°C for 20 h | Bombyx mori |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Bombyx mori | the transcription of the enzyme decreases in the adult stage | down |
| Bombyx mori | the transcription of the enzyme increases significantly during the prepupal to pupal stage | up |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.3 | - |
4-nitrophenyl alpha-N-acetyl-D-galactosaminide | at pH 7.0 and 30°C | Bombyx mori |
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